Gene expression profiling in healthy newborns from diverse localities of the Czech Republic
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
29602183
DOI
10.1002/em.22184
Knihovny.cz E-zdroje
- Klíčová slova
- PM2.5, air pollution, benzo[a]pyrene, cord blood, microarray, prenatal exposure,
- MeSH
- fetální krev účinky léků metabolismus MeSH
- látky znečišťující vzduch toxicita MeSH
- lidé MeSH
- monitorování životního prostředí * MeSH
- novorozenec MeSH
- polycyklické aromatické uhlovodíky toxicita MeSH
- regulace genové exprese účinky léků genetika MeSH
- stanovení celkové genové exprese metody MeSH
- těhotenství MeSH
- vystavení vlivu životního prostředí MeSH
- znečištění ovzduší škodlivé účinky MeSH
- Check Tag
- lidé MeSH
- novorozenec MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- látky znečišťující vzduch MeSH
- polycyklické aromatické uhlovodíky MeSH
Prenatal exposure to air pollution is associated with intrauterine growth restriction and low birth weight. Gene expression changes in newborns in relation to air pollution have not been sufficiently studied. We analyzed whole genome expression in cord blood leukocytes of 202 newborns from diverse localities of the Czech Republic, differing among other factors in levels of air pollution: the district of Karvina (characterized by higher concentration of air pollutants) and Ceske Budejovice (lower air pollution levels). We aimed to identify differentially expressed genes (DEGs) and pathways in relation to locality and concentration of air pollutants. We applied the linear model to identify the specific DEGs and the correlation analysis, to investigate the relationship between the concentrations of air pollutants and gene expression data. An analysis of biochemical pathways and gene set enrichment was also performed. In general, we observed modest changes of gene expression, mostly attributed to the effect of the locality. The highest number of DEGs was found in samples from the district of Karvina. A pathway analysis revealed a deregulation of processes associated with cell growth, apoptosis or cellular homeostasis, immune response-related processes or oxidative stress response. The association between concentrations of air pollutants and gene expression changes was weak, particularly for samples collected in Karvina. In summary, as we did not find a direct effect of exposure to air pollutants, we assume that the general differences in the environment, rather than actual concentrations of individual pollutants, represent a key factor affecting gene expression changes at delivery. Environ. Mol. Mutagen. 59:401-415, 2018. © 2018 Wiley Periodicals, Inc.
Czech Technical University Prague Czech Republic
Faculty of Health and Social Studies University of South Bohemia Ceske Budejovice Czech Republic
Institute of Experimental Medicine of the Czech Academy of Sciences Prague Czech Republic
School of Public Health University at Albany Rensselaer New York
Citace poskytuje Crossref.org
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