Monodisperse magnetic poly(glycidyl methacrylate) microspheres for isolation of autoantibodies with affinity for the 46 kDa form of unconventional Myo1C present in autoimmune patients
Jazyk angličtina Země Rakousko Médium electronic
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
LQ1604
Ministerstvo Školství, Mládeže a Tělovýchovy - International
PubMed
29687337
DOI
10.1007/s00604-018-2807-5
PII: 10.1007/s00604-018-2807-5
Knihovny.cz E-zdroje
- Klíčová slova
- Affinity chromatography, Autoantibody, Autoimmune disease marker, Functionalization, Immunoglobulin M, Magnetic microspheres, Multiple sclerosis, p46/Myo1C protein,
- MeSH
- autoimunitní nemoci imunologie MeSH
- autoprotilátky krev chemie imunologie izolace a purifikace MeSH
- kyseliny polymethakrylové chemie MeSH
- lidé MeSH
- magnety chemie MeSH
- mikrosféry * MeSH
- molekulová hmotnost MeSH
- myosin typu I chemie imunologie MeSH
- roztroušená skleróza imunologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- autoprotilátky MeSH
- kyseliny polymethakrylové MeSH
- MYO1C protein, human MeSH Prohlížeč
- myosin typu I MeSH
- polyglycidyl methacrylate MeSH Prohlížeč
Monodisperse nonmagnetic macroporous poly(glycidyl methacrylate) (PGMA) microspheres were synthesized by multistep swelling polymerization of glycidyl methacrylate, ethylene dimethacrylate and 2-[(methoxycarbonyl)methoxy]ethyl methacrylate (MCMEMA). This was followed (a) by ammonolysis to modify the microspheres with amino groups, and (b) by incorporation of iron oxide (γ-Fe2O3) into the pores to render the particles magnetic. The resulting porous and magnetic microspheres were characterized by scanning and transmission electron microscopy (SEM and TEM), atomic absorption and Fourier transform infrared spectroscopy (AAS and FTIR), elemental analysis, vibrating magnetometry, mercury porosimetry and Brunauer-Emmett-Teller adsorption/desorption isotherms. The microspheres are meso- and macroporous, typically 5 μm in diameter, contain 0.9 mM · g-1 of amino groups and 14 wt.% of iron according to elemental analysis and AAS, respectively. The particles were conjugated to p46/Myo1C protein, a potential biomarker of autoimmune diseases, to isolate specific autoantibodies in the blood of patients suffering from multiple sclerosis (MS). The p46/Myo1C loaded microspheres are shown to enable the preconcentration of minute quantities of specific immunoglobulins prior to their quantification via SDS-PAGE. The immunoglobulin M (IgM) with affinity to Myo1C was detected in MS patients. Graphical abstract Monodisperse magnetic poly(glycidyl methacrylate) microspheres were synthesized, conjugated with 46 kDa form of unconventional Myo1C protein (p46/Myo1C) via carbodiimide (DIC) chemistry, and specific autoantibodies isolated from blood of multiple sclerosis (MS) patients; immunoglobulin M (IgM) level increased in MS patients.
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