Monodisperse magnetic poly(glycidyl methacrylate) microspheres for isolation of autoantibodies with affinity for the 46 kDa form of unconventional Myo1C present in autoimmune patients
Language English Country Austria Media electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
LQ1604
Ministerstvo Školství, Mládeže a Tělovýchovy - International
PubMed
29687337
DOI
10.1007/s00604-018-2807-5
PII: 10.1007/s00604-018-2807-5
Knihovny.cz E-resources
- Keywords
- Affinity chromatography, Autoantibody, Autoimmune disease marker, Functionalization, Immunoglobulin M, Magnetic microspheres, Multiple sclerosis, p46/Myo1C protein,
- MeSH
- Autoimmune Diseases immunology MeSH
- Autoantibodies blood chemistry immunology isolation & purification MeSH
- Polymethacrylic Acids chemistry MeSH
- Humans MeSH
- Magnets chemistry MeSH
- Microspheres * MeSH
- Molecular Weight MeSH
- Myosin Type I chemistry immunology MeSH
- Multiple Sclerosis immunology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Autoantibodies MeSH
- Polymethacrylic Acids MeSH
- MYO1C protein, human MeSH Browser
- Myosin Type I MeSH
- polyglycidyl methacrylate MeSH Browser
Monodisperse nonmagnetic macroporous poly(glycidyl methacrylate) (PGMA) microspheres were synthesized by multistep swelling polymerization of glycidyl methacrylate, ethylene dimethacrylate and 2-[(methoxycarbonyl)methoxy]ethyl methacrylate (MCMEMA). This was followed (a) by ammonolysis to modify the microspheres with amino groups, and (b) by incorporation of iron oxide (γ-Fe2O3) into the pores to render the particles magnetic. The resulting porous and magnetic microspheres were characterized by scanning and transmission electron microscopy (SEM and TEM), atomic absorption and Fourier transform infrared spectroscopy (AAS and FTIR), elemental analysis, vibrating magnetometry, mercury porosimetry and Brunauer-Emmett-Teller adsorption/desorption isotherms. The microspheres are meso- and macroporous, typically 5 μm in diameter, contain 0.9 mM · g-1 of amino groups and 14 wt.% of iron according to elemental analysis and AAS, respectively. The particles were conjugated to p46/Myo1C protein, a potential biomarker of autoimmune diseases, to isolate specific autoantibodies in the blood of patients suffering from multiple sclerosis (MS). The p46/Myo1C loaded microspheres are shown to enable the preconcentration of minute quantities of specific immunoglobulins prior to their quantification via SDS-PAGE. The immunoglobulin M (IgM) with affinity to Myo1C was detected in MS patients. Graphical abstract Monodisperse magnetic poly(glycidyl methacrylate) microspheres were synthesized, conjugated with 46 kDa form of unconventional Myo1C protein (p46/Myo1C) via carbodiimide (DIC) chemistry, and specific autoantibodies isolated from blood of multiple sclerosis (MS) patients; immunoglobulin M (IgM) level increased in MS patients.
See more in PubMed
Anal Chem. 2006 Aug 15;78(16):5627-32 PubMed
Nature. 1970 Aug 15;227(5259):680-5 PubMed
Chem Commun (Camb). 2017 Aug 3;53(63):8902-8905 PubMed
J Colloid Interface Sci. 2018 Feb 15;512:600-608 PubMed
Biochem Biophys Rep. 2015 Dec 03;5:175-179 PubMed
Small. 2013 May 27;9(9-10):1450-66 PubMed
J Chromatogr A. 2017 Aug 25;1512:1-8 PubMed
Biosci Rep. 2017 Apr 28;37(2): PubMed
Biomaterials. 2009 Sep;30(27):4601-9 PubMed
ACS Appl Mater Interfaces. 2017 May 10;9(18):15265-15273 PubMed
Chem Rev. 2008 Jun;108(6):2064-110 PubMed
