Cholesterol-modified superporous poly(2-hydroxyethyl methacrylate) scaffolds for tissue engineering
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19500833
DOI
10.1016/j.biomaterials.2009.05.007
PII: S0142-9612(09)00496-7
Knihovny.cz E-resources
- MeSH
- Cholesterol metabolism MeSH
- Hydrogels chemistry MeSH
- Carbodiimides chemistry MeSH
- Microscopy, Confocal MeSH
- Rats MeSH
- Methacrylates chemistry pharmacology MeSH
- Mesenchymal Stem Cells cytology drug effects MeSH
- Microscopy, Electron, Scanning MeSH
- Cell Count MeSH
- Porosity drug effects MeSH
- Solutions MeSH
- Spectrophotometry, Ultraviolet MeSH
- Tissue Engineering * MeSH
- Tissue Scaffolds chemistry MeSH
- Cell Survival drug effects MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cholesterol MeSH
- Hydrogels MeSH
- hydroxyethyl methacrylate MeSH Browser
- Carbodiimides MeSH
- Methacrylates MeSH
- Solutions MeSH
Modifications of poly(2-hydroxyethyl methacrylate) (PHEMA) with cholesterol and laminin have been developed to design scaffolds that promote cell-surface interaction. Cholesterol-modified superporous PHEMA scaffolds have been prepared by the bulk radical copolymerization of 2-hydroxyethyl methacrylate (HEMA), cholesterol methacrylate (CHLMA) and the cross-linking agent ethylene dimethacrylate (EDMA) in the presence of ammonium oxalate crystals to introduce interconnected superpores in the matrix. With the aim of immobilizing laminin (LN), carboxyl groups were also introduced to the scaffold by the copolymerization of the above monomers with 2-[(methoxycarbonyl)methoxy]ethyl methacrylate (MCMEMA). Subsequently, the MCMEMA moiety in the resulting hydrogel was hydrolyzed to [2-(methacryloyloxy)ethoxy]acetic acid (MOEAA), and laminin was immobilized via carbodiimide and N-hydroxysulfosuccinimide chemistry. The attachment, viability and morphology of mesenchymal stem cells (MSCs) were evaluated on both nonporous and superporous laminin-modified as well as laminin-unmodified PHEMA and poly(2-hydroxyethyl methacrylate-co-cholesterol methacrylate) P(HEMA-CHLMA) hydrogels. Neat PHEMA and laminin-modified PHEMA (LN-PHEMA) scaffolds facilitated MSC attachment, but did not support cell spreading and proliferation; the viability of the attached cells decreased with time of cultivation. In contrast, MSCs spread and proliferated on P(HEMA-CHLMA) and LN-P(HEMA-CHLMA) hydrogels.
References provided by Crossref.org
Biomaterials and Magnetic Stem Cell Delivery in the Treatment of Spinal Cord Injury
Life on magnets: stem cell networking on micro-magnet arrays
