Cholesterol-modified superporous poly(2-hydroxyethyl methacrylate) scaffolds for tissue engineering
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
19500833
DOI
10.1016/j.biomaterials.2009.05.007
PII: S0142-9612(09)00496-7
Knihovny.cz E-zdroje
- MeSH
- cholesterol metabolismus MeSH
- hydrogely chemie MeSH
- karbodiimidy chemie MeSH
- konfokální mikroskopie MeSH
- krysa rodu Rattus MeSH
- methakryláty chemie farmakologie MeSH
- mezenchymální kmenové buňky cytologie účinky léků MeSH
- mikroskopie elektronová rastrovací MeSH
- počet buněk MeSH
- poréznost účinky léků MeSH
- roztoky MeSH
- spektrofotometrie ultrafialová MeSH
- tkáňové inženýrství * MeSH
- tkáňové podpůrné struktury chemie MeSH
- viabilita buněk účinky léků MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- cholesterol MeSH
- hydrogely MeSH
- hydroxyethyl methacrylate MeSH Prohlížeč
- karbodiimidy MeSH
- methakryláty MeSH
- roztoky MeSH
Modifications of poly(2-hydroxyethyl methacrylate) (PHEMA) with cholesterol and laminin have been developed to design scaffolds that promote cell-surface interaction. Cholesterol-modified superporous PHEMA scaffolds have been prepared by the bulk radical copolymerization of 2-hydroxyethyl methacrylate (HEMA), cholesterol methacrylate (CHLMA) and the cross-linking agent ethylene dimethacrylate (EDMA) in the presence of ammonium oxalate crystals to introduce interconnected superpores in the matrix. With the aim of immobilizing laminin (LN), carboxyl groups were also introduced to the scaffold by the copolymerization of the above monomers with 2-[(methoxycarbonyl)methoxy]ethyl methacrylate (MCMEMA). Subsequently, the MCMEMA moiety in the resulting hydrogel was hydrolyzed to [2-(methacryloyloxy)ethoxy]acetic acid (MOEAA), and laminin was immobilized via carbodiimide and N-hydroxysulfosuccinimide chemistry. The attachment, viability and morphology of mesenchymal stem cells (MSCs) were evaluated on both nonporous and superporous laminin-modified as well as laminin-unmodified PHEMA and poly(2-hydroxyethyl methacrylate-co-cholesterol methacrylate) P(HEMA-CHLMA) hydrogels. Neat PHEMA and laminin-modified PHEMA (LN-PHEMA) scaffolds facilitated MSC attachment, but did not support cell spreading and proliferation; the viability of the attached cells decreased with time of cultivation. In contrast, MSCs spread and proliferated on P(HEMA-CHLMA) and LN-P(HEMA-CHLMA) hydrogels.
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