High performance liquid chromatography column efficiency enhancement by zero dead volume recycling and practical approach using park and recycle arrangement
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
29706399
DOI
10.1016/j.chroma.2018.03.058
PII: S0021-9673(18)30385-6
Knihovny.cz E-zdroje
- Klíčová slova
- Circulation chromatography, Closed loop recycling, Peak recycling, Recycle system, Sample recycler,
- MeSH
- benzenové deriváty chemie izolace a purifikace MeSH
- polycyklické sloučeniny chemie izolace a purifikace MeSH
- protiproudá chromatografie MeSH
- recyklace * MeSH
- stereoizomerie MeSH
- vysokoúčinná kapalinová chromatografie přístrojové vybavení metody MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- benzenové deriváty MeSH
- helicenes MeSH Prohlížeč
- polycyklické sloučeniny MeSH
A new instrumental approach to recycling HPLC is described. The concept is based on fast reintroduction of incremental peak sections back onto the separation column. The re-circulation is performed within a closed loop containing only the column and two synchronized switching valves. By having HPLC pump out of the cycle, the method minimizes peak broadening due to dead volume. As a result the efficiency is dramatically increased allowing for the most demanding analytical applications. In addition, a parking loop is employed for temporary storage of analytes from the middle section of the separated mixture prior to their recycling.
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