Using a portable Raman spectrometer to detect carotenoids of halophilic prokaryotes in synthetic inclusions in NaCl, KCl, and sulfates
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články
Grantová podpora
2221/15
Israel Science Foundation
UNCE/SCI/006
Center for Geosphere Dynamics, UNCE, Charles University
17-04270S
Grantová Agentura České Republiky
PubMed
29725727
DOI
10.1007/s00216-018-1098-3
PII: 10.1007/s00216-018-1098-3
Knihovny.cz E-zdroje
- Klíčová slova
- Carotenoids, Halophilic prokaryotes, Inclusions, Miniature Raman spectrometer, Salts,
- MeSH
- Bacteroidetes chemie MeSH
- chlorid draselný chemie MeSH
- chlorid sodný chemie MeSH
- Halobacterium salinarum chemie MeSH
- Halorubrum chemie MeSH
- karotenoidy analýza MeSH
- limita detekce MeSH
- Ramanova spektroskopie přístrojové vybavení MeSH
- sírany chemie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- chlorid draselný MeSH
- chlorid sodný MeSH
- karotenoidy MeSH
- sírany MeSH
Cell suspensions of the haloarchaea Halorubrum sodomense and Halobacterium salinarum and the extremely halophilic bacterium Salinibacter ruber (Bacteroidetes) in saturated solutions of chlorides and sulfates (NaCl, KCl, MgSO4·7H2O, K2SO4, and (NH4)Al(SO4)2·12H2O) were left to evaporate to produce micrometric inclusions in laboratory-grown crystals. Raman spectra of these pinkish inclusions were obtained using a handheld Raman spectrometer with green excitation (532 nm). This portable instrument does not include any microscopic tool. Acceptable Raman spectra of carotenoids were obtained in the range of 200-4000 cm-1. This detection achievement was related to the mode of illumination and collection of scattered light as well as due to resonance Raman enhancement of carotenoid signals under green excitation. The position of diagnostic Raman carotenoid bands corresponds well to those specific carotenoids produced by a given halophile. To our best knowledge, this is the first study of carotenoids included in the laboratory in crystalline chlorides and sulfates, using a miniature portable Raman spectrometer. Graphical abstract ᅟ.
Citace poskytuje Crossref.org
Microbial colonization of gypsum: from the fossil record to the present day