PREMISE OF THE STUDY: Polymorphic microsatellite markers were developed to study genetic diversity, population structure, and dispersal strategies of the highly invasive species Rumex alpinus (Polygonaceae). METHODS AND RESULTS: Fifteen polymorphic microsatellite loci were developed using a 454 sequencing approach and used to genotype 72 individuals from six populations in Austria and France. All markers were polymorphic in at least one investigated population, and the number of alleles ranged from one to four alleles per locus. Observed and expected heterozygosity ranged from 0.06 to 1.0 and from 0.18 to 0.72, respectively. CONCLUSIONS: These 15 markers will be useful for characterizing dispersal strategies and gene flow assessment between R. alpinus populations and other, often weedy Rumex species.

Department of Botany Faculty of Science Charles University Prague Benátská 2 128 01 Prague Czech Republic

Institute of Botany of the Czech Academy of Sciences Zámek 1 252 43 Průhonice Czech Republic

See more in PubMed

Červenková, Z. , and Münzbergová Z.. 2009. Susceptibility of the landscape of the Giant Mountains, Czech Republic, to invasion by Rumex alpinus In Pyšek P. and Pergl J. [eds.], Biological invasions: Towards a synthesis. Neobiota 8: 75–85.

Klimeš, L. 1992. The clone architecture of Rumex alpinus (Polygonaceae). Oikos 63: 402–409.

Lodhi, M. A. , Ye G. N., Weeden N. F., and Reisch B. I.. 1994. A simple and efficient method for DNA extraction from grapevine cultivars and Vitis species. Plant Molecular Biology Reporter 12: 6–13.

Malausa, T. , Gilles A., Meglécz E., Blanquart H., Duthoy S., Costedoat C., Dubut V., et al. 2011. High‐throughput microsatellite isolation through 454 GS‐FLX Titanium pyrosequencing of enriched DNA libraries. Molecular Ecology Resources 11: 638–644. PubMed

Meglécz, E. , Costedoat C., Dubut V., Gilles A., Malausa T., Pech N., and Martin J. F.. 2010. QDD: A user‐friendly program to choose microsatellite markers and design primers from large sequencing projects. Bioinformatics 26: 403–404. PubMed

Peakall, R. , and Smouse P. E.. 2012. GenAlEx 6.5: Genetic analysis in Excel. Population genetic software for teaching and research–an update. Bioinformatics 28: 2537–2539. PubMed PMC

Raycheva, T. , Denev I., and Dimitrova D.. 2013. Taxonomic relationships of selected Bulgarian species from Rumex subg. Rumex (Polygonaceae) based on ISSR markers. Phytologia Balcanica 19: 29–37.

Rousset, F. 2008. GENEPOP'007: A complete re‐implementation of the GENEPOP software for Windows and Linux. Molecular Ecology Resources 8: 103–106. PubMed

Schuelke, M. 2000. An economic method for the fluorescent labeling of PCR fragments. Nature Biotechnology 18: 233–234. PubMed

Šťastná, P. , Klimeš L., and Klimešová J.. 2010. Biological flora of Central Europe: Rumex alpinus L. Perspectives in Plant Ecology, Evolution and Systematics 12: 67–79.

Origin and genetic variability of populations of the invasive plant Rumex alpinus L. in the Giant (Krkonoše) Mountains