Two strains of Gram-positive cocci were isolated from viscera of common voles (Microtus arvalis Pallas) with generalized Brucella microti infection in the Czech Republic. Biochemical features and phylogenetic analysis based on 16S rRNA gene sequences showed that the strains are representatives of the genus Staphylococcus and assigned Staphylococcus muscae as the nearest relative. A detailed characterization done by ribotyping, rpoB and hsp60 gene sequencing, whole-cell protein analysis and rep-PCR using the (GTG)(5) primer differentiated the two strains from all described staphylococci. DNA-DNA hybridization with the type strain of S. muscae demonstrated that the two strains should be considered as members of a novel species (26.8 % reassociation). The two analysed strains were found to be coagulase-negative, novobiocin-susceptible, oxidase-negative cultures, phenotypically close to one another, but showing differences in ribotype profiles. The major fatty acids were iso-C(15 : 0), iso-C(17 : 0), anteiso-C(15 : 0), C(18 : 2 )omega6,9c/anteiso-C(18 : 0), C(18 : 0) and C(18 : 1) omega9c. MK-7 was the predominant isoprenoid quinone, with minor amounts of MK-6 and MK-8. The polar lipid profile was composed of the major lipids diphosphatidylglycerol and phosphatidylglycerol and several unknown lipids. These results proved that the two isolates represent a novel staphylococcal species. The name proposed for this novel taxon is Staphylococcus microti sp. nov.; the type strain is 4005-LJ(m)(T) (=CCM 4903(T) =CCUG 55861(T) =DSM 22147(T)).

Culture Collection University of Göteborg Department of Clinical Bacteriology Guldhedsgatan 10 SE 413 46 Göteborg Sweden

Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH Inhoffenstrasse 7b D 38124 Braunschweig Germany

Institut für Bakteriologie Mykologie und Hygiene Veterinärmedizinische Universität Veterinärplatz 1 A 1210 Wien Austria

Institute of Vertebrate Biology Academy of Sciences Květná 8 CZ 603 65 Brno Czech Republic

Masaryk University Institute of Experimental Biology Czech Collection of Microorganisms Tvrdého 14 CZ 602 00 Brno Czech Republic

Masaryk University Institute of Experimental Biology Department of Genetics and Molecular Biology Kotlářská2 CZ 611 37 Brno Czech Republic

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Staphylococcus ratti sp. nov. Isolated from a Lab Rat

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