Staphylococcus microti sp. nov., isolated from the common vole (Microtus arvalis)
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Arvicolinae * MeSH
- DNA, Bacterial genetics MeSH
- Phylogeny MeSH
- Fatty Acids chemistry metabolism MeSH
- Molecular Sequence Data MeSH
- Rodent Diseases microbiology MeSH
- DNA, Ribosomal genetics MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Staphylococcal Infections microbiology veterinary MeSH
- Staphylococcus classification genetics isolation & purification MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Bacterial MeSH
- Fatty Acids MeSH
- DNA, Ribosomal MeSH
- RNA, Ribosomal, 16S MeSH
Two strains of Gram-positive cocci were isolated from viscera of common voles (Microtus arvalis Pallas) with generalized Brucella microti infection in the Czech Republic. Biochemical features and phylogenetic analysis based on 16S rRNA gene sequences showed that the strains are representatives of the genus Staphylococcus and assigned Staphylococcus muscae as the nearest relative. A detailed characterization done by ribotyping, rpoB and hsp60 gene sequencing, whole-cell protein analysis and rep-PCR using the (GTG)(5) primer differentiated the two strains from all described staphylococci. DNA-DNA hybridization with the type strain of S. muscae demonstrated that the two strains should be considered as members of a novel species (26.8 % reassociation). The two analysed strains were found to be coagulase-negative, novobiocin-susceptible, oxidase-negative cultures, phenotypically close to one another, but showing differences in ribotype profiles. The major fatty acids were iso-C(15 : 0), iso-C(17 : 0), anteiso-C(15 : 0), C(18 : 2 )omega6,9c/anteiso-C(18 : 0), C(18 : 0) and C(18 : 1) omega9c. MK-7 was the predominant isoprenoid quinone, with minor amounts of MK-6 and MK-8. The polar lipid profile was composed of the major lipids diphosphatidylglycerol and phosphatidylglycerol and several unknown lipids. These results proved that the two isolates represent a novel staphylococcal species. The name proposed for this novel taxon is Staphylococcus microti sp. nov.; the type strain is 4005-LJ(m)(T) (=CCM 4903(T) =CCUG 55861(T) =DSM 22147(T)).
References provided by Crossref.org
Staphylococcus ratti sp. nov. Isolated from a Lab Rat
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