A novel three-fluorophore system as a ratiometric sensor for multiple protease detection
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
PubMed
29796477
DOI
10.1039/c8cc01731j
Knihovny.cz E-resources
- MeSH
- Aminocoumarins chemical synthesis chemistry radiation effects MeSH
- Chymotrypsin analysis MeSH
- Enzyme Assays MeSH
- Phenylalanine analogs & derivatives chemistry MeSH
- Fluoresceins chemical synthesis chemistry radiation effects MeSH
- Fluorescence MeSH
- Fluorescent Dyes chemical synthesis chemistry radiation effects MeSH
- Hydrolysis MeSH
- Lysine analogs & derivatives chemistry MeSH
- Fluorescence Resonance Energy Transfer MeSH
- Rhodamines chemical synthesis chemistry radiation effects MeSH
- Drug Stability MeSH
- Trypsin analysis MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Aminocoumarins MeSH
- Chymotrypsin MeSH
- Phenylalanine MeSH
- Fluoresceins MeSH
- Fluorescent Dyes MeSH
- Lysine MeSH
- Rhodamines MeSH
- Trypsin MeSH
A synthetic three-fluorophore system with two enzymatically cleavable linkers has been developed for the simultaneous detection of two proteases in a mixture. The probe was designed to afford single excitation/triple emission ratiometric detection through a fluorescence change during the cleavage of a peptide linker. The developed assays were verified for trypsin and chymotrypsin as the model enzymes.
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