Rapid identification of Streptomyces tetracycline producers by MALDI-TOF mass spectrometry
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
- Keywords
- MALDI-TOF MS, Tetracycline, streptomyces,
- MeSH
- Databases, Factual MeSH
- Humans MeSH
- High-Throughput Screening Assays methods MeSH
- Software MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization * MeSH
- Streptomyces isolation & purification metabolism MeSH
- Tetracycline chemistry isolation & purification metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Tetracycline MeSH
The main objective of this study was using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for assembling of DSM (German Collection of Microorganisms) Streptomyces spectral database and identification of wild Streptomyces cultures, which were clustered by MALDI-TOF Biotyper OC software as well as for teracycline detection by observing of obtained spectra using flexAnalysis software. Production of tetracycline was confirmed by thin-layer chromatography. Presence of tetracycline mass spectrum was verified by several tetracycline producers (Streptomyces aureofaciens LMG 5968, S. aureofaciens 84/25, and S. aureofaciens BMK) and by pure tetracycline mass. Our results showed that it is possible to use MALDI-TOF MS for identification of tetracycline producers within Streptomyces genera by several easy steps. The purpose of this study was to establish cheap and quick detection of tetracycline producers.
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