Flexible Alkyne-Linked Thymidine Phosphoramidites and Triphosphates for Chemical or Polymerase Synthesis and Fast Postsynthetic DNA Functionalization through Copper-Catalyzed Alkyne-Azide 1,3-Dipolar Cycloaddition
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- alkyny chemie MeSH
- azidy MeSH
- cykloadiční reakce MeSH
- DNA MeSH
- katalýza MeSH
- měď MeSH
- molekulární struktura MeSH
- organofosforové sloučeniny MeSH
- polyfosfáty MeSH
- thymidin MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- alkyny MeSH
- azidy MeSH
- DNA MeSH
- měď MeSH
- organofosforové sloučeniny MeSH
- phosphoramidite MeSH Prohlížeč
- polyfosfáty MeSH
- thymidin MeSH
- triphosphoric acid MeSH Prohlížeč
Two alternative flexible alkyne-linked thymine nucleosides (propargyl-diethylene glycol- or undecyn-linked 5-hydroxymethyluracil derivatives), as well as their phosphoramidites and triphosphates, were designed and synthesized. The nucleoside 3'- O-phosphoramidites were successfully incorporated into oligonucleotides on a solid support, whereas the nucleoside triphosphates served as good substrates for polymerase synthesis of modified DNA, which underwent fast and efficient copper-catalyzed alkyne-azide 1,3-dipolar cycloaddition (CuAAC) reactions.
Citace poskytuje Crossref.org
