Determination of 8-iso-prostaglandin F(2alpha) in exhaled breath condensate using combination of immunoseparation and LC-ESI-MS/MS
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem, validační studie
PubMed
18373964
DOI
10.1016/j.jchromb.2008.02.019
PII: S1570-0232(08)00138-4
Knihovny.cz E-zdroje
- MeSH
- dechové testy * MeSH
- dinoprost analogy a deriváty analýza MeSH
- hmotnostní spektrometrie s elektrosprejovou ionizací metody MeSH
- kalibrace MeSH
- lidé MeSH
- reprodukovatelnost výsledků MeSH
- senzitivita a specificita MeSH
- tandemová hmotnostní spektrometrie metody MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- validační studie MeSH
- Názvy látek
- 8-epi-prostaglandin F2alpha MeSH Prohlížeč
- dinoprost MeSH
Rapid and precise method for the determination of 8-iso-prostaglandin F(2alpha), an essential marker of the oxidative stress, in exhaled breath condensate (EBC) was developed. The protocol consisted of stable isotope dilution, immunoseparation combined with selective and sensitive LC-ESI-MS/MS operated in multiple reaction monitoring (MRM) mode. The imprecision of the developed method was below 8.8%, the parameter of mean inaccuracy was determined as <9.6% (0-250pg of 8-iso-prostaglandin F(2alpha)/ml EBC). The limit of detection (LOD) was 1 pg/ml EBC and limit of quantification (LOQ) 5 pg/ml EBC. A significant difference in 8-iso-prostaglandin F(2alpha) content between the group of asbestosis patients and healthy volunteers was found.
Citace poskytuje Crossref.org
Markers of Oxidative Stress in the Exhaled Breath Condensate of Workers Handling Nanocomposites
Multimarker screening of oxidative stress in aging
