Determination of 8-iso-prostaglandin F(2alpha) in exhaled breath condensate using combination of immunoseparation and LC-ESI-MS/MS
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't, Validation Study
PubMed
18373964
DOI
10.1016/j.jchromb.2008.02.019
PII: S1570-0232(08)00138-4
Knihovny.cz E-resources
- MeSH
- Breath Tests * MeSH
- Dinoprost analogs & derivatives analysis MeSH
- Spectrometry, Mass, Electrospray Ionization methods MeSH
- Calibration MeSH
- Humans MeSH
- Reproducibility of Results MeSH
- Sensitivity and Specificity MeSH
- Tandem Mass Spectrometry methods MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Validation Study MeSH
- Names of Substances
- 8-epi-prostaglandin F2alpha MeSH Browser
- Dinoprost MeSH
Rapid and precise method for the determination of 8-iso-prostaglandin F(2alpha), an essential marker of the oxidative stress, in exhaled breath condensate (EBC) was developed. The protocol consisted of stable isotope dilution, immunoseparation combined with selective and sensitive LC-ESI-MS/MS operated in multiple reaction monitoring (MRM) mode. The imprecision of the developed method was below 8.8%, the parameter of mean inaccuracy was determined as <9.6% (0-250pg of 8-iso-prostaglandin F(2alpha)/ml EBC). The limit of detection (LOD) was 1 pg/ml EBC and limit of quantification (LOQ) 5 pg/ml EBC. A significant difference in 8-iso-prostaglandin F(2alpha) content between the group of asbestosis patients and healthy volunteers was found.
References provided by Crossref.org
Markers of Oxidative Stress in the Exhaled Breath Condensate of Workers Handling Nanocomposites
Multimarker screening of oxidative stress in aging
