Nowadays, regenerative and reparative medicine has grown in popularity. Dental stem cells are easily accessible source of adult stem cells. They can be harvested by a tooth extraction or spontaneous deciduous tooth exfoliation. They have to be isolated, expanded and stored until time they would be needed for individual stem cell therapy. Cryopreservation is both a short-term and long-term storage of tissues or cells at sub-zero temperatures. There are several methods of cryopreservation requiring different technologies. The objective of this review is to compare them and highlight their advantages and disadvantages.

Department of Dentistry Charles University Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové Czech Republic

References provided by Crossref.org

Innovative Approach in the Cryogenic Freezing Medium for Mesenchymal Stem Cells