The broad fish tapeworm Dibothriocephalus latus is a causative agent of human food-borne disease called diphyllobothriosis. Medical importance, scattered geographical distribution and unknown origin of D. latus in Europe and North America make this species to be an interesting model for population genetics. Microsatellite markers were originally designed by library screening using NGS approach and validated as tools for future studies on population genetics of D. latus. Out of 122 candidates selected after NGS analysis, 110 yielded PCR products of the expected size, and in 78 of them, a declared repetitive motif was confirmed by Sanger sequencing. After the fragment analysis, six loci were proved to be polymorphic and tested for observed (Ho) and expected (He) heterozygosity, and deviations from Hardy-Weinberg equilibrium (HWE). They promise future application in studies on genetic interrelationships, origin and migratory routes of this medically important emerging tapeworm.

Biology Centre CAS Institute of Parasitology Branišovská 31 37005 České Budějovice Czech Republic

Biology Centre CAS Institute of Parasitology Branišovská 31 37005 České Budějovice Czech Republic; Faculty of Science University of South Bohemia Branišovská 1160 31 37005 České Budějovice Czech Republic

Faculty of Natural Sciences Comenius University Mlynská dolina B 2 84215 Bratislava Slovakia

Faculty of Natural Sciences Comenius University Mlynská dolina B 2 84215 Bratislava Slovakia; Medirex a s Galvaniho 17 C P O Box 143 82016 Bratislava Slovakia

Institute of Parasitology Slovak Academy of Sciences Hlinkova 3 04001 Košice Slovakia

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Unique genetic structure of the human tapeworm Dibothriocephalus latus from the Alpine lakes region - a successful adaptation?

Molecular cytogenetic analysis of a triploid population of the human broad tapeworm, Dibothriocephalus latus (Diphyllobothriidea)