Dibothriocephalus latus is the most frequent causative agent of fish-borne zoonosis (diphyllobothriosis) in Europe, where it is currently circulating mainly in the Alpine lakes region (ALR) and Russia. Three mitochondrial genes (cox1, cob and nad3) and 6 microsatellite loci were analysed to determine how is the recently detected triploidy/parthenogenesis in tapeworms from ALR displayed at the DNA level. A geographically distant population from the Krasnoyarsk Reservoir in Russia (RU-KR) was analysed as a comparative population. One or 2 alleles of each microsatellite locus was detected in plerocercoids from RU-KR, corresponding to the microsatellite pattern of a diploid organism. In contrast, 1–3 alleles were observed in tapeworms from ALR, in accordance with their triploidy. The high diversity of mitochondrial haplotypes in D. latus from RU-KR implied an original and relatively stable population, but the identical structure of mitochondrial genes of tapeworms from ALR was probably a consequence of a bottleneck typical of introduced populations. These results indicated that the diploid/sexually reproducing population from RU-KR was ancestral, located within the centre of the distribution of the species, and the triploid/parthenogenetically reproducing subalpine population was at the margin of the distribution. The current study revealed the allelic structure of the microsatellite loci in the triploid tapeworm for the first time.

Biology Centre CAS Institute of Parasitology Branišovská 31 37005 České Budějovice Czech Republic

Department of Microbiology and Virology Faculty of Natural Sciences Comenius University in Bratislava Ilkovičova 6 84215 Bratislava Slovakia

Department of Veterinary Medical Sciences University of Bologna Via Tolara di Sopra 50 40064 Ozzano Emilia BO Italy

Faculty of Science University of South Bohemia Branišovská 1760 37005 České Budějovice Czech Republic

Institute of Parasitology Slovak Academy of Sciences Hlinkova 3 04001 Košice Slovakia

Krasnoyarsk Branch of the Russian Federal Research Institute of Fisheries and Oceanography 'VNIRO' Parizhskoi Kommuny 33 660097 Krasnoyarsk Russia

Medirex a s Galvaniho 17 C P O Box 143 82016 Bratislava Slovakia

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