Modified Biosensor for Cholinesterase Inhibitors with Guinea Green B as the Color Indicator
Jazyk angličtina Země Švýcarsko Médium electronic
Typ dokumentu časopisecké články
Grantová podpora
VI20152018024
Ministerstvo Vnitra České Republiky
PubMed
30181477
PubMed Central
PMC6164029
DOI
10.3390/bios8030081
PII: bios8030081
Knihovny.cz E-zdroje
- Klíčová slova
- Guinea Green B, biosensor of cholinesterase inhibitors, chemical warfare agents, enzymatic reaction, visual evaluation,
- MeSH
- biosenzitivní techniky metody MeSH
- chemické bojové látky analýza MeSH
- cholinesterasové inhibitory analýza MeSH
- kolorimetrie metody MeSH
- lissaminová zelená barviva chemie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- chemické bojové látky MeSH
- cholinesterasové inhibitory MeSH
- guinea green B MeSH Prohlížeč
- lissaminová zelená barviva MeSH
Colorimetric biosensors of cholinesterase inhibitors are ideal for fast, reliable, and very simple detection of agents in air, in water, and on surfaces. This paper describes an innovation of the Czech Detehit biosensor, which is based on a biochemical enzymatic reaction visualized by using Ellman's reagent as a chromogenic indicator. The modification basically consists of a much more distinct color response of the biosensor, attained through optimization of the reaction system by using Guinea Green B as the indicator. The performance of the modified biosensor was verified on the chemical warfare agents (sarin, soman, cyclosarin, and VX) in water. The detection limits ascertained visually (with the naked eye) were about 0.001 µg/mL in water (exposure time 60 s, inhibition efficiency 25%).
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