"Candidatus Phytoplasma prunorum" (CPp) is a highly destructive phytopathogenic agent in many stone fruit-growing regions in Europe and the surrounding countries. In this work, we focused on documenting entire bacterial community in the phloem tissues of 60 stone fruit trees. Nested PCR and two real-time PCR assays were used to select CPp-positive (group A) and CPp-negative samples (group B). Afterwards, high-throughput amplicon sequencing was performed to assess bacterial community compositions in phloem tissues. The bacterial composition in phloem tissue consisted of 118 distinct genera, represented mainly by Pseudomonas, Acinetobacter, Methylobacterium, Sphingomonas, and Rhizobium. Statistics showed that CPp influenced the bacterial composition of infected plants (group A) and that the bacterial community depended on the geographical origin of the sample. This is the first work focusing on an analysis of the influence of CPp on the bacteria coexisting in the phloem tissues of stone fruit trees.

Department of Biology Faculty of Science University of Hradec Králové Rokitanského 62 500 03 Hradec Králové Czech Republic

Department of Fruit Growing Mendel University in Brno Valtická 337 691 44 Lednice Czech Republic

Department of Plant Protection University of Agriculture in Krakow 29 Listopada 54 31 425 Krakow Poland

Laboratory of Environmental Microbiology Institute of Microbiology of the CAS Vídeňská 1083 14220 Praha 4 Czech Republic

Mendeleum Institute of Genetics Mendel University in Brno Valtická 334 691 44 Lednice Czech Republic

Research and Breeding Institute of Pomology Holovousy Ltd Holovousy 129 508 01 Hořice Czech Republic

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