Enzyme linked receptor protein signaling pathway is one of the ontology groups that are highly up-regulated in porcine oocytes before in vitro maturation
Jazyk angličtina Země Itálie Médium print
Typ dokumentu časopisecké články
PubMed
30334402
PII: 5
Knihovny.cz E-zdroje
- MeSH
- IVM techniky * MeSH
- kumulární buňky cytologie MeSH
- oocyty cytologie růst a vývoj metabolismus MeSH
- prasata MeSH
- signální transdukce genetika MeSH
- stanovení celkové genové exprese MeSH
- transkriptom * MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Before being able to fully participate in the processes associated with its function as a female gamete, the oocyte needs to undergo a range of changes to achieve its mature form. These morphological, biochemical and metabolomic processes are induced by the somatic tissues surrounding the oocyte, through the expression of specific transcription and growth factors. The maturation of the oocyte is highly important for the proceedings that lead to successful fertilization, early embryonic development and implantation. Domestic pigs were used as models for our study, with the cumulus-oocyte complexes obtained from the ovaries that were recovered at slaughter. After shedding of the cumulus, oocytes were assessed with BCB test, with the viable ones chosen to undergo in vitro maturation. With the use of expression microarrays, we analyzed gene expression before and after IVM and detected major changes in both genes that were proven to be associated with oocyte maturation before (FOS, VEGFA, CHRDL1, TGFBR3, FST, INSR, ID1, TXNIP, SMAD4, MAP3K1, EIF2AK3 and KIT) and genes not previously linked with reproduction associated processes (MYO1E, PHIP, KLF10 and SHOC2). All the genes were briefly described, with consideration of possible involvement of the newly discovered elements of the transcriptome in the process of oocyte maturation.
Department of Anatomy Poznan University of Medical Sciences Poznan Poland
Department of Histology and Embryology Poznan University of Medical Sciences Poznan Poland
Department of Human Morphology and Embryology Wroclaw University of Medical Sciences Wroclaw Poland
Department of Pathophysiology Poznań University of Medical Sciences Poznan Poland
Division of Anatomy and Histology University of Zielona Góra Zieloga Góra Poland
Division of Perinatology and Women's Diseases Poznan University of Medical Sciences Poznan Poland
Veterinary Center Nicolaus Copernicus University in Toruń Torun Poland