Effects of selenate and red Se-nanoparticles on the photosynthetic apparatus of Nicotiana tabacum
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
Odkazy
PubMed
30374728
DOI
10.1007/s11120-018-0599-4
PII: 10.1007/s11120-018-0599-4
Knihovny.cz E-zdroje
- Klíčová slova
- Chlorophyll fluorescence transients, Chloroplast thylakoid membranes, Circular dichroism, Electron microscopy, Nicotiana tabacum, Selenate and Se-nanoparticles, Small-angle neutron scattering,
- MeSH
- chlorofyl metabolismus MeSH
- chloroplasty metabolismus MeSH
- cirkulární dichroismus MeSH
- fotosyntéza fyziologie MeSH
- kyselina selenová metabolismus MeSH
- nanočástice chemie MeSH
- tabák metabolismus MeSH
- tylakoidy metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- chlorofyl MeSH
- kyselina selenová MeSH
Selenium (Se) is a natural trace element, which shifts its action in a relatively narrow concentration range from nutritional role to toxicity. Although it has been well established that in plants chloroplasts are among the primary targets, the mechanism of toxicity on photosynthesis is not well understood. Here, we compared selenate and red-allotrope elemental selenium nanoparticles (red nanoSe) in in vitro tobacco cultures to investigate their effects on the structure and functions of the photosynthetic machinery. Selenate at 10 mg/L concentration retarded plant growth; it also led to a decreased chlorophyll content, accompanied with an increase in the carotenoid-to-chlorophyll ratio. Structural examinations of the photosynthetic machinery, using electron microscopy, small-angle neutron scattering and circular dichroism spectroscopy, revealed significant perturbation in the macro-organization of the pigment-protein complexes and sizeable shrinkage in the repeat distance of granum thylakoid membranes. As shown by chlorophyll a fluorescence transient measurements, these changes in the ultrastructure were associated with a significantly diminished photosystem II activity and a reduced performance of the photosynthetic electron transport, and an enhanced capability of non-photochemical quenching. These changes in the structure and function of the photosynthetic apparatus explain, at least in part, the retarded growth of plantlets in the presence of 10 mg/L selenate. In contrast, red nanoSe, even at 100 mg/L and selenate at 1 mg/L, exerted no negative effect on the growth of plantlets and affected only marginally the thylakoid membrane ultrastructure and the photosynthetic functions.
