The functional significance of having two nuclei in a cell is unknown. Having two stores of genetic material may be advantageous for cell growth. Nuclear protein import is at a critical juncture in the cell to modify cell growth. This study addressed the potential for differential nuclear protein import in two nuclei of the same cell. Isolated adult rat cardiomyocytes were microinjected with an exogenous fluorescent protein conjugated with nuclear localization amino acid sequences to facilitate nuclear import and its detection. Our results demonstrate the rate of nuclear protein import was not significantly different between the two nuclei in the same cell. These data demonstrate that the two nuclei are functionally similar in a binucleated cardiomyocyte, at least as far as nucleocytoplasmic transport is concerned.

Institute of Cardiovascular Sciences Albrechtsen Research Centre St Boniface Hospital Winnipeg Canada; Anatomy and Cell Biology Rady Faculty of Health Sciences University of Manitoba Winnipeg MB Canada

Institute of Cardiovascular Sciences Albrechtsen Research Centre St Boniface Hospital Winnipeg Canada; Department of Physiology and Pathophysiology University of Manitoba Winnipeg MB Canada

Institute of Physiology Czech Academy of Sciences Prague Czech Republic; Institute of Cardiovascular Sciences Albrechtsen Research Centre St Boniface Hospital Winnipeg Canada; Department of Physiology and Pathophysiology University of Manitoba Winnipeg MB Canada

Citace poskytuje Crossref.org

Epitranscriptomic regulation in fasting hearts: implications for cardiac health