Do different nuclei in a binucleated cardiomyocyte have different rates of nuclear protein import?
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
30502349
DOI
10.1016/j.yjmcc.2018.08.030
PII: S0022-2828(18)30337-7
Knihovny.cz E-resources
- Keywords
- Binuclear cell, Cardiomyocytes, Nuclear protein import, Nucleocytoplasmic trafficking, Nucleus,
- MeSH
- Active Transport, Cell Nucleus MeSH
- Cell Nucleus metabolism MeSH
- Myocytes, Cardiac metabolism MeSH
- Microinjections MeSH
- Rats, Sprague-Dawley MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The functional significance of having two nuclei in a cell is unknown. Having two stores of genetic material may be advantageous for cell growth. Nuclear protein import is at a critical juncture in the cell to modify cell growth. This study addressed the potential for differential nuclear protein import in two nuclei of the same cell. Isolated adult rat cardiomyocytes were microinjected with an exogenous fluorescent protein conjugated with nuclear localization amino acid sequences to facilitate nuclear import and its detection. Our results demonstrate the rate of nuclear protein import was not significantly different between the two nuclei in the same cell. These data demonstrate that the two nuclei are functionally similar in a binucleated cardiomyocyte, at least as far as nucleocytoplasmic transport is concerned.
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