One-step nucleic acid amplification vs ultrastaging in the detection of sentinel lymph node metastasis in endometrial cancer patients
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články
Grantová podpora
00669806
Faculty Hospital in Pilsen
PubMed
30508294
DOI
10.1002/jso.25322
Knihovny.cz E-zdroje
- Klíčová slova
- cytokeratin 19, micrometastasis, one-step nucleic acid amplification assay,
- MeSH
- adenokarcinom z jasných buněk genetika sekundární chirurgie MeSH
- biopsie sentinelové lymfatické uzliny MeSH
- dospělí MeSH
- keratin-19 genetika MeSH
- lidé středního věku MeSH
- lidé MeSH
- lymfatické metastázy MeSH
- mikrometastázy MeSH
- míra přežití MeSH
- nádorové biomarkery genetika MeSH
- nádory endometria genetika patologie chirurgie MeSH
- následné studie MeSH
- nukleové kyseliny analýza genetika MeSH
- peroperační doba MeSH
- prognóza MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- sentinelová uzlina patologie chirurgie MeSH
- serózní cystadenokarcinom genetika sekundární chirurgie MeSH
- techniky amplifikace nukleových kyselin metody MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Názvy látek
- keratin-19 MeSH
- nádorové biomarkery MeSH
- nukleové kyseliny MeSH
BACKGROUND AND OBJECTIVES: Utilisation of the one-step nucleic acid amplification (OSNA) molecular biology method for the detection of the metastatic involvement of sentinel lymph nodes (SLNs) in endometrial cancer (EC) patients. A comparison with histopathological ultrastaging and a description of the clinical consequences. METHODS: Surgically treated EC patients underwent detection of SLNs. Nodes greater than 5 mm were cut into sections 2-mm thick parallel to the short axis of the node. Odd sections were examined according to the OSNA method, while even ones according to an appropriate ultrastaging protocol. Nodes less than or equal to 5 mm were cut into halves along the longitudinal axis with one half examined according to the OSNA method and the other half by ultrastaging. RESULTS: Fifty-eight patients were included and 135 SLNs were acquired. Both ultrastaging and OSNA agreed on 116 results. According to the OSNA method, 20.69% more patients were classified into International Federation of Gynecology and Obstetrics (FIGO) stage III. When comparing the results of the OSNA method to the conclusions of ultrastaging as a reference method, sensitivity of 90.9%, specificity of 85.5% and concordance of 85.9% were attained. CONCLUSIONS: The results of the OSNA method showed a higher frequency of detection of micrometastases and included 20.69% more patients into FIGO stage III.
Biomedical Centre Faculty of Medicine in Pilsen Charles University Pilsen Czech Republic
Department of Biology Faculty of Medicine in Pilsen Charles University Pilsen Czech Republic
Sikl's Department of Pathology University Hospital Pilsen Charles University Prague Czech Republic
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