One-step nucleic acid amplification vs ultrastaging in the detection of sentinel lymph node metastasis in endometrial cancer patients
Language English Country United States Media print-electronic
Document type Comparative Study, Journal Article
Grant support
00669806
Faculty Hospital in Pilsen
PubMed
30508294
DOI
10.1002/jso.25322
Knihovny.cz E-resources
- Keywords
- cytokeratin 19, micrometastasis, one-step nucleic acid amplification assay,
- MeSH
- Adenocarcinoma, Clear Cell genetics secondary surgery MeSH
- Sentinel Lymph Node Biopsy MeSH
- Adult MeSH
- Keratin-19 genetics MeSH
- Middle Aged MeSH
- Humans MeSH
- Lymphatic Metastasis MeSH
- Neoplasm Micrometastasis MeSH
- Survival Rate MeSH
- Biomarkers, Tumor genetics MeSH
- Endometrial Neoplasms genetics pathology surgery MeSH
- Follow-Up Studies MeSH
- Nucleic Acids analysis genetics MeSH
- Intraoperative Period MeSH
- Prognosis MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Sentinel Lymph Node pathology surgery MeSH
- Cystadenocarcinoma, Serous genetics secondary surgery MeSH
- Nucleic Acid Amplification Techniques methods MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Names of Substances
- Keratin-19 MeSH
- Biomarkers, Tumor MeSH
- Nucleic Acids MeSH
BACKGROUND AND OBJECTIVES: Utilisation of the one-step nucleic acid amplification (OSNA) molecular biology method for the detection of the metastatic involvement of sentinel lymph nodes (SLNs) in endometrial cancer (EC) patients. A comparison with histopathological ultrastaging and a description of the clinical consequences. METHODS: Surgically treated EC patients underwent detection of SLNs. Nodes greater than 5 mm were cut into sections 2-mm thick parallel to the short axis of the node. Odd sections were examined according to the OSNA method, while even ones according to an appropriate ultrastaging protocol. Nodes less than or equal to 5 mm were cut into halves along the longitudinal axis with one half examined according to the OSNA method and the other half by ultrastaging. RESULTS: Fifty-eight patients were included and 135 SLNs were acquired. Both ultrastaging and OSNA agreed on 116 results. According to the OSNA method, 20.69% more patients were classified into International Federation of Gynecology and Obstetrics (FIGO) stage III. When comparing the results of the OSNA method to the conclusions of ultrastaging as a reference method, sensitivity of 90.9%, specificity of 85.5% and concordance of 85.9% were attained. CONCLUSIONS: The results of the OSNA method showed a higher frequency of detection of micrometastases and included 20.69% more patients into FIGO stage III.
Biomedical Centre Faculty of Medicine in Pilsen Charles University Pilsen Czech Republic
Department of Biology Faculty of Medicine in Pilsen Charles University Pilsen Czech Republic
Sikl's Department of Pathology University Hospital Pilsen Charles University Prague Czech Republic
References provided by Crossref.org
