The current study reports data on antioxidant, antimicrobial and neutrophil-modulating activities of different polyphenolic preparations from black chokeberry fruits: crude extract, purified extract standardized to 20% and 40% anthocyanins, and proanthocyanidins; as well as pure compounds (chlorogenic acid, cyanidin-3-O-galactoside, epicatechin, rutin and quercetin) present in black chokeberries. Minor phenolic components - quercetin and epicatechin showed the highest ORAC and TRAP antioxidant activity. Given the amount of individual phenolics in the fruits, proanthocyanidins are the major contributor to antioxidant activity of fresh black chokeberries. Studied polyphenols and preparations had no effect on the spontaneous chemiluminescence (CL) of human neutrophils and only mild effect on PMA-activated CL. Greater effects were observed on OZP-activated CL, being statistically significant (p < 0.05) for quercetin and rutin. The antimicrobial activity test against 10 pathogens showed that black chokeberry proanthocyanidins are the most potent antimicrobial agents in the fruit.

Institute of Biophysics of the Czech Academy of Sciences Kralovopolska 135 612 65 Brno Czech Republic; Department of Animal Physiology and Immunology Institute of Experimental Biology Masaryk University Kotlarska 2 611 37 Brno Czech Republic

Laboratory of Biologically Active Substances Institute of Organic Chemistry with Centre of Phytochemistry Bulgarian Academy of Sciences 139 Ruski Blvd 4000 Plovdiv Bulgaria

University of Food Technologies 26 Maritsa Blvd 4002 Plovdiv Bulgaria

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