• MeSH
• extrakt ze semen vinné révy * terapeutické užití   MeSH
• fytoterapie MeSH
• léčivé rostliny MeSH
• lidé MeSH
• proantokyanidiny terapeutické užití   MeSH
• rostlinné extrakty MeSH
• stárnutí MeSH
• Vitis MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• populární práce MeSH

The mycotoxin zearalenone (ZEA) in food and feed seriously harms human and animal health. How to reduce its toxicity is an important direction of current research on food safety. This study aim to assess the effects of procyanidins (PC) on cell apoptosis caused by ZEA and to clarify the role of Nrf2 in the process. Swine testicle (ST) cells were treated with ZEA (57.5 μmol/L) and/or PC (10 mg/L) for 24 h. Cell viability was detected by CCK-8 assay. Cell apoptosis and the level of ROS were detected by flow cytometry. The expression levels of mRNA and protein was detected by qRT-PCR and western blotting. Our results showed that ZEA reduced the antioxidant capacity of the ST cells, induced the cell apoptosis and inhibited the gene and protein expression of Nrf2 and its downstream genes (ho-1,nqo1), while PC improved the cell antioxidant capacity, reduced the degree of ZEA-induced cell apoptosis and promoted the gene and protein expression of Nrf2 and its downstream genes. However, when the Nrf2 small molecule inhibitor ML385 was added, the ability of PC to inhibit ZEA-induced cell apoptosis and promote the expression of Nrf2 and its downstream genes were decreased. Our results demonstrated that ZEA induced oxidative stress and apoptosis of ST cells, which were alleviated by PC intervention via activating Nrf2 signaling pathway. This finding of this study provided a molecular basis for the clinical application of PC to prevent ZEN-caused reproductive toxicity.

• MeSH
• antioxidancia metabolismus   farmakologie   MeSH
• apoptóza MeSH
• faktor 2 související s NF-E2 genetika   metabolismus   MeSH
• oxidační stres MeSH
• prasata MeSH
• proantokyanidiny * farmakologie   MeSH
• reaktivní formy kyslíku metabolismus   MeSH
• signální transdukce MeSH
• testis metabolismus   MeSH
• zearalenon * metabolismus   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

In Italy a particularly valuable chestnut is "Marrone di Roccadaspide", a protected geographical indication (PGI) product, deriving from a Castanea sativa cultivar, typical of Salerno province in Campania region. As chestnut industrial processes yield a large amount of shell by-products, in this study the possibility to retrain this waste food as potential source of bioactives was investigated. The ability of "Marrone di Roccadaspide" shell MeOH extract to modulate the pro-inflammatory transcriptional factor NF-κB after LPS stimulation, along with the antioxidant activity by a cell-based in vitro test, were evaluated. To correlate the NF-κB inhibition (67.67% at 5 μg/mL) and the strong antioxidant activity to the chemical composition, an analytical approach based on LC-ESI/LTQOrbitrap/MS/MSn along with NMR characterization of isolated compounds was developed. The identification of hydrolysable and condensed tannins, along with flavonoids, phenol glucosides, ellagic acid derivatives, and triterpenoids was accomplished. The most representative compounds were quantitatively analyzed by LC-ESI/QTrap/MS/MS, showing bartogenic acid as the compound occurring in the highest amount (103.08 mg/100 g shells). With the aim to explore the possibility to employ chestnut shells as suitable source of bioactives for the preparation of functional ingredients, the chemical composition and the antioxidant activity of "eco-friendly" extracts (EtOH and EtOH:H2O 7:3) was finally evaluated, showing a high superimposability of the EtOH:H2O (7:3) extract to the MeOH extract.

• MeSH
• antioxidancia analýza   MeSH
• Fagaceae chemie   MeSH
• fenoly analýza   MeSH
• flavonoidy analýza   MeSH
• glukosidy analýza   MeSH
• hodnotící studie jako téma MeSH
• hydrolyzovatelné taniny analýza   MeSH
• kalibrace MeSH
• kyselina ellagová analýza   MeSH
• lidé MeSH
• NF-kappa B metabolismus   MeSH
• ořechy chemie   MeSH
• proantokyanidiny analýza   MeSH
• proliferace buněk MeSH
• racionalizace * MeSH
• reaktivní formy kyslíku MeSH
• rostlinné extrakty analýza   MeSH
• tandemová hmotnostní spektrometrie MeSH
• taniny analýza   MeSH
• triterpeny analýza   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Itálie MeSH

In Japan, adzuki bean is cooked with rice. During the cooking, the colour of rice becomes pale red. It is postulated that the red pigment is produced from procyanidins and that the ingestion of red rice causes the production of nitric oxide (•NO) in the stomach by reacting with salivary nitrite. The increase in colour intensity accompanied the decrease in the amounts of procyanidins, suggesting the conversion of procyanidins into the red pigment during the cooking. In addition, the red pigment combined with rice strongly. The red-coloured rice produced •NO by reacting with nitrite in artificial gastric juice, and the amounts were dependent on the contents of procyanidins and the equivalents. It is suggested that although adzuki procyanidins were oxidised during cooking with rice, procyanidins and the equivalents bound to rice still have the ability to produce bioactive •NO in the stomach using nitrite in mixed whole saliva.

• MeSH
• anthokyaniny metabolismus   MeSH
• biflavonoidy metabolismus   MeSH
• dusitany metabolismus   MeSH
• katechin metabolismus   MeSH
• kyselina askorbová metabolismus   MeSH
• kyselina dehydroaskorbová metabolismus   MeSH
• lidé MeSH
• oxid dusnatý metabolismus   MeSH
• oxidace-redukce MeSH
• peptidylprolylisomerasa Pin1 MeSH
• proantokyanidiny metabolismus   MeSH
• rýže (rod) metabolismus   MeSH
• škrob MeSH
• sliny MeSH
• vaření * MeSH
• vigna chemie   MeSH
• vysoká teplota MeSH
• žaludeční šťáva metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Japonsko MeSH

Combining high-carbohydrate food with polyphenol-rich food is a possible way of producing slowly digestible starch with beneficial health properties. In Japan, non-glutinous and glutinous rice are cooked with adzuki bean and the colour of the cooked rice is pale red. In this article, we show that (1) the red colour of rice could be attributed to the oxidation of adzuki bean procyanidins, (2) pancreatin-induced starch digestion of the red-coloured non-glutinous rice was slower than white rice and (3) the digestion of amylose and potato starch but not amylopectin became slower by heating with procyanidin B2. Furthermore, the rate of starch digestion of red-coloured rice was not affected by nitrite treatment under simulated gastric conditions. The above results show that procyanidins could bind to amylose independent of the starch source by heating and could suppress starch digestion by α-amylase in the intestine.

• MeSH
• alfa-amylasy MeSH
• amylopektin metabolismus   MeSH
• amylosa metabolismus   MeSH
• biflavonoidy MeSH
• katechin MeSH
• lidé MeSH
• pankreatin metabolismus   MeSH
• proantokyanidiny metabolismus   MeSH
• rýže (rod) * MeSH
• trávení * MeSH
• vaření MeSH
• vigna * MeSH
• vysoká teplota MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Japonsko MeSH

Cinnamtannin B-1 (CNB-1) is a naturally occurring trimeric A-type proanthocyanidin contained in several plants such as cinnamon (Cinnamomum zeylanicum). It is considered to be a potent antioxidant. The protective effect of CNB-1 against oxidative stress was assessed in red deer epididymal sperm incubated at 37 °C. Cryopreserved sperm from six stags were thawed, pooled and extended to 400 × 106 sperm/ml in BGM (bovine gamete medium). After being aliquoted, the samples were supplemented with different concentrations of CNB-1 (0, 0.1, 1, 10 and 100 μg/mL), with or without induced oxidative stress (100 μM Fe2+/ascorbate). The samples were evaluated after 0, 2 and 4 h of incubation at 37 °C. This experiment was replicated six times. Spermmotility (CASA), viability, mitochondrial membrane potential, acrosomal status, lipoperoxidation (C11 BODIPY 581/591), intracellular reactive oxygen species (ROS) production and DNA status (TUNEL) were assessed. After 4 h of incubation, CNB-1 prevented the deleterious effects of oxidative stress, thus improved sperm progressivity and velocity (P<0.05). Furthermore, 1 and 10 μM CNB-1 improved sperm linearity, even when compared to those samples that had not been subjected to oxidative stress (P<0.05). The greatest concentration, 100 μM, prevented sperm lipoperoxidation and reduced ROS production in samples subjected to oxidative stress.

• MeSH
• antioxidancia farmakologie   MeSH
• fragmentace DNA MeSH
• motilita spermií účinky léků   MeSH
• oxidační stres účinky léků   MeSH
• peroxidace lipidů MeSH
• proantokyanidiny farmakologie   MeSH
• reaktivní formy kyslíku metabolismus   MeSH
• spermie účinky léků   MeSH
• uchování spermatu veterinární   MeSH
• viabilita buněk účinky léků   MeSH
• vysoká zvěř * MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

Compared to most other alcoholic beverages, the shelf life of beer is much more limited due to its instability in the bottle. That instability is most likely to appear as turbidity (haze), even sedimentation, during storage. The haze in beer is mostly caused by colloidal particles formed by interactions between proteins and polyphenols within the beer. Therefore, beers are usually stabilized by removing at least one of these components. We developed and constructed a Saccharomyces cerevisiae strain with a proline-rich QPF peptide attached to the cell wall, using the C-terminal anchoring domain of α-agglutinin. The QPF peptide served to bind polyphenols during fermentation and, thus, to decrease their concentration. Strains displaying QPF were able to bind about twice as much catechin and epicatechin as a control strain displaying only the anchoring domain. All these experiments were done with model solutions. Depending on the concentration of yeast, uptake of polyphenols was 1.7-2.5 times higher. Similarly, the uptake of proanthocyanidins was increased by about 20 %. Since the modification of yeasts with QPF did not affect their fermentation performance under laboratory conditions, the display of QPF appears to be an approach to increase the stability of beer.

• MeSH
• biflavonoidy metabolismus   MeSH
• katechin metabolismus   MeSH
• pivo mikrobiologie   MeSH
• polyfenoly metabolismus   MeSH
• potravinářská mikrobiologie MeSH
• proantokyanidiny metabolismus   MeSH
• Saccharomyces cerevisiae metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH

Procyanidins, contained in many products abundant in human diet, exhibit high biological activity. However, this activity has not been fully explained at cellular and molecular levels. In this study, we determine the mechanism of interaction of procyanidin B3 with model lipid membrane. This mechanism was established on the basis of changes induced by B3 in the physical properties of lipid bilayer. The changes were investigated using steady state and time-resolved fluorescence, DSC, and FTIR. We show that procyanidin B3 causes changes in the arrangement of the polar heads of lipids, order of their acyl chains and the main lipid phase transition temperature. Furthermore, its presence in the membrane leads to a reduction in membrane dipole potential. Procyanidin B3 is anchored to membrane via hydrogen bonds formed between its OH groups and the PO2- and CO groups of lipids, causing changes in both hydrophilic and hydrophobic regions of the membrane.

• MeSH
• 2-naftylamin analogy a deriváty   chemie   MeSH
• biflavonoidy chemie   MeSH
• diferenciální skenovací kalorimetrie MeSH
• dihydropyridiny chemie   MeSH
• dimyristoylfosfatidylcholin chemie   MeSH
• fluorescenční spektrometrie MeSH
• hydrofobní a hydrofilní interakce MeSH
• katechin chemie   MeSH
• laurany chemie   MeSH
• lipidové dvojvrstvy chemie   MeSH
• proantokyanidiny chemie   MeSH
• spektroskopie infračervená s Fourierovou transformací MeSH
• teplota MeSH
• termodynamika MeSH
• vodíková vazba MeSH
• změna skupenství MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

In this study, biochemical profile of fruits of 9 Sorbus genotypes was analyzed. The content of total sugars ranged from 69.7 g/kg ('Titan') to 217.5 g/kg (Sorbus torminalis) and total organic acids from 17.7 g/kg ('Businka') to 40.2 g/kg (S. torminalis). The highest content of total anthocyanins had 'Burka' (871 mg/kg FW) and 'Businka' (856 mg/kg FW). Quercetin derivatives represented more than 95% of total flavonols. 'Alaja krupnaja' had 3.5- to 29-fold higher rutin content than other analyzed genotypes. S. torminalis fruits had the greatest diversity of isorhamnetin and kaempferol derivatives. Chlorogenic acid was the major hydroxycinnamic acid and its share was 33% to 73% of total analyzed hydoxycinnamic acid derivatives. The richest in chlorogenic acid were 'Krasavica' and 'Alaja krupnaja' fruits. Cultivar 'Businka' had the highest content of epicatechin (40.7 mg/kg) and neochlorogenic acid (1061 mg/kg). Different procyanidin oligomers were detected among flavanols in Sorbus fruits. The highest content of total flavanols was measured in 'Alaja Krupnaja' fruits. Cultivars 'Krasavica' (84.5 mg/kg) and 'Burka' (85.1 mg/kg) had 1.2- to 6.9-fold higher amount of total carotenoids. 'Businka' was highlighted as the richest in total tannin and phenolic contents (3768 mg GAE/kg) and consequently, it had the highest antioxidant activity (57.6 mM TE/kg FW). Being abundant in polyphenolics, some extracts of Sorbus genotypes, for example, 'Businka,' 'Burka,' and 'Alaja krupnaja' could serve as valuable resource of bioactive compounds to food and pharmaceutical industries.

• MeSH
• anthokyaniny analýza   MeSH
• antioxidancia analýza   MeSH
• biflavonoidy analýza   MeSH
• fenoly analýza   MeSH
• flavonoly analýza   MeSH
• genotyp * MeSH
• karotenoidy analýza   MeSH
• katechin analýza   MeSH
• kempferoly analýza   MeSH
• kyselina chinová analogy a deriváty   analýza   MeSH
• kyselina chlorogenová analogy a deriváty   analýza   MeSH
• ovoce chemie   MeSH
• polyfenoly analýza   MeSH
• proantokyanidiny analýza   MeSH
• quercetin analogy a deriváty   analýza   MeSH
• rostlinné extrakty chemie   MeSH
• Sorbus chemie   MeSH
• Publikační typ
• časopisecké články MeSH

• Klíčová slova
• náplasti LENI, fyziologická regulační medicína, MD-Tissue, MD-Small Joints,
• MeSH
• Achillova šlacha * MeSH
• chronická bolest MeSH
• chronická nemoc MeSH
• Eucalyptus MeSH
• fytoterapie MeSH
• Harpagophytum MeSH
• injekce MeSH
• kolagen * aplikace a dávkování   terapeutické užití   MeSH
• komplementární terapie MeSH
• lidé středního věku MeSH
• lidé MeSH
• okluzivní ošetření rány MeSH
• proantokyanidiny terapeutické užití   MeSH
• rostlinné extrakty MeSH
• výsledek terapie MeSH
• zánět * farmakoterapie   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• kazuistiky MeSH