Antifungal activity of analogues of antimicrobial peptides isolated from bee venoms against vulvovaginal Candida spp
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
30753486
DOI
10.1093/femsyr/foz013
PII: 5315757
Knihovny.cz E-resources
- Keywords
- Candida albicans, Candida tropicalis, antifungal peptides, biofilm eradication, biofilm inhibition, vulvovaginal candidiasis,
- MeSH
- Amphotericin B pharmacology MeSH
- Antifungal Agents chemical synthesis pharmacology MeSH
- Biofilms drug effects MeSH
- Candida drug effects MeSH
- Human Umbilical Vein Endothelial Cells drug effects MeSH
- Hyphae drug effects MeSH
- Candidiasis, Vulvovaginal microbiology MeSH
- Antimicrobial Cationic Peptides chemical synthesis pharmacology MeSH
- Cells, Cultured MeSH
- Humans MeSH
- Microbial Sensitivity Tests MeSH
- Bee Venoms chemistry MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Amphotericin B MeSH
- Antifungal Agents MeSH
- Antimicrobial Cationic Peptides MeSH
- Bee Venoms MeSH
Candida albicans is the main causative agent of vulvovaginal candidiasis (VVC), a common mycosis in women, relapses of which are difficult to manage due to biofilm formation. This study aimed at developing novel non-toxic compounds active against Candida spp. biofilms. We synthesised analogues of natural antifungal peptides LL-III (LL-III/43) and HAL-2 (peptide VIII) originally isolated from bee venoms and elucidated their structures by nuclear magnetic resonance spectroscopy. The haemolytic, cytotoxic, antifungal and anti-biofilm activities of LL-III/43 and peptide VIII were then tested. LL-III/43 and VIII showed moderate cytotoxicity to HUVEC-2 cells and had comparable inhibitory activity against C. albicans and non-albicans spp. The lowest minimum inhibitory concentration (MIC90) of LL-III/43 was observed towards Candida tropicalis (0.8 µM). That was 8-fold lower than that of antimycotic amphotericin B. Both peptides can be used to inhibit Candida spp. bio film f ormation. Biofilm inhibitory concentrations (BIC50) ranged from 0.9 to 58.6 µM and biofilm eradication concentrations (BEC50) for almost all tested Candida spp. strains ranged from 12.8 to 200 µM. Als o pro ven were the peptides' abilities to reduce the area colonised by biofilms , inhibit hyphae formation and permeabilise cell membranes in biofil ms . LL-III/43 and VIII are promising candidates for further development as therapeutics against VVC.
Faculty of Mathematics and Physics Charles University Ke Karlovu 3 121 16 Prague 2 Czech Republic
University of Chemistry and Technology Prague Technická 5 166 28 Prague 6 Czech Republic
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