Antifungal activity of analogues of antimicrobial peptides isolated from bee venoms against vulvovaginal Candida spp
Jazyk angličtina Země Velká Británie, Anglie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
30753486
DOI
10.1093/femsyr/foz013
PII: 5315757
Knihovny.cz E-zdroje
- Klíčová slova
- Candida albicans, Candida tropicalis, antifungal peptides, biofilm eradication, biofilm inhibition, vulvovaginal candidiasis,
- MeSH
- amfotericin B farmakologie MeSH
- antifungální látky chemická syntéza farmakologie MeSH
- biofilmy účinky léků MeSH
- Candida účinky léků MeSH
- endoteliální buňky pupečníkové žíly (lidské) účinky léků MeSH
- hyfy účinky léků MeSH
- kandidóza vulvovaginální mikrobiologie MeSH
- kationické antimikrobiální peptidy chemická syntéza farmakologie MeSH
- kultivované buňky MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- včelí jedy chemie MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- amfotericin B MeSH
- antifungální látky MeSH
- kationické antimikrobiální peptidy MeSH
- včelí jedy MeSH
Candida albicans is the main causative agent of vulvovaginal candidiasis (VVC), a common mycosis in women, relapses of which are difficult to manage due to biofilm formation. This study aimed at developing novel non-toxic compounds active against Candida spp. biofilms. We synthesised analogues of natural antifungal peptides LL-III (LL-III/43) and HAL-2 (peptide VIII) originally isolated from bee venoms and elucidated their structures by nuclear magnetic resonance spectroscopy. The haemolytic, cytotoxic, antifungal and anti-biofilm activities of LL-III/43 and peptide VIII were then tested. LL-III/43 and VIII showed moderate cytotoxicity to HUVEC-2 cells and had comparable inhibitory activity against C. albicans and non-albicans spp. The lowest minimum inhibitory concentration (MIC90) of LL-III/43 was observed towards Candida tropicalis (0.8 µM). That was 8-fold lower than that of antimycotic amphotericin B. Both peptides can be used to inhibit Candida spp. bio film f ormation. Biofilm inhibitory concentrations (BIC50) ranged from 0.9 to 58.6 µM and biofilm eradication concentrations (BEC50) for almost all tested Candida spp. strains ranged from 12.8 to 200 µM. Als o pro ven were the peptides' abilities to reduce the area colonised by biofilms , inhibit hyphae formation and permeabilise cell membranes in biofil ms . LL-III/43 and VIII are promising candidates for further development as therapeutics against VVC.
Faculty of Mathematics and Physics Charles University Ke Karlovu 3 121 16 Prague 2 Czech Republic
University of Chemistry and Technology Prague Technická 5 166 28 Prague 6 Czech Republic
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