Intralaboratory comparison of analytical methods for quantification of major phytocannabinoids
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články
Grantová podpora
RO0419
Ministerstvo Zemědělství
PubMed
30895348
DOI
10.1007/s00216-019-01760-y
PII: 10.1007/s00216-019-01760-y
Knihovny.cz E-zdroje
- Klíčová slova
- Flame ionization detector, Gas chromatography, Liquid chromatography, Mass spectrometry, Phytocannabinoids,
- MeSH
- Cannabis chemie MeSH
- kanabinoidy analýza MeSH
- laboratoře organizace a řízení MeSH
- limita detekce MeSH
- plamínková ionizace metody MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí metody MeSH
- reprodukovatelnost výsledků MeSH
- tandemová hmotnostní spektrometrie metody MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Názvy látek
- kanabinoidy MeSH
This study compares alternative approaches for analyzing phytocannabinoids in different plant materials. Three chromatographic analytical methods (ultra-high-performance liquid chromatography with tandem mass spectrometric detection and gas chromatography with mass spectrometric and flame ionization detection) were evaluated regarding selectivity, sensitivity, analytical accuracy, and precision. The performance of the methods was compared and all three methods were demonstrated to be appropriate tools for analyzing phytocannabinoids in cannabis. Gas chromatography coupled with mass spectrometric detection showed slightly better accuracy in determining phytocannabinoid acids, which are often difficult to quantify owing to their limited stability. Aspects of sample preparation, such as material homogenization and extraction, were also considered. A single ultrasonic-assisted ethanolic extraction of dried and powdered plant samples of cannabis was shown to be exhaustive for extracting the samples prior to analysis.
Citace poskytuje Crossref.org
