Cytopathological features of secretory carcinoma of salivary glands and ancillary techniques in its diagnostics: impact of new Milan system for reporting salivary gland cytopathology
Language English Country Denmark Media print-electronic
Document type Journal Article
Grant support
Ministry of Education, Youth and Sports of the Czech Republic
PubMed
30942913
DOI
10.1111/apm.12950
Knihovny.cz E-resources
- Keywords
- ETV6, FNA, Milan system, Salivary glands, mammary analog secretory carcinoma, secretory carcinoma,
- MeSH
- Cytodiagnosis methods MeSH
- Adult MeSH
- Immunohistochemistry methods MeSH
- Carcinoma diagnosis metabolism pathology MeSH
- Middle Aged MeSH
- Humans MeSH
- Young Adult MeSH
- Biomarkers, Tumor metabolism MeSH
- Salivary Gland Neoplasms diagnosis metabolism pathology MeSH
- ETS Translocation Variant 6 Protein MeSH
- S100 Proteins metabolism MeSH
- Proto-Oncogene Proteins c-ets metabolism MeSH
- Repressor Proteins metabolism MeSH
- Aged MeSH
- Salivary Glands metabolism pathology MeSH
- Biopsy, Fine-Needle methods MeSH
- Vimentin metabolism MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Young Adult MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Biomarkers, Tumor MeSH
- S100 Proteins MeSH
- Proto-Oncogene Proteins c-ets MeSH
- Repressor Proteins MeSH
- Vimentin MeSH
Secretory carcinoma (SC) of salivary glands is a newly described low-grade malignancy characterized by the presence of ETV6 rearrangement. Only a few cases and very small series with cytomorphology were reported so far. Six cases of fine-needle aspirations (FNAs) from afterward histologically, immunohistochemically and genetically confirmed SCs were retrieved from the archives of the authors. Ancillary immunocytochemistry (ICC) and translocation detection were performed on cell blocks (CBs). All aspirates were sufficiently cellular and cells were arranged in more or less cohesive groups with only mild nuclear polymorphism. The cytoplasm was eosinophilic, granulated and vacuolated, especially in CBs. Secretory material within the microcystic spaces was periodic acid-Schiff (PAS) positive. Triple positivity of immunomarkers S-100 protein, mammaglobin and vimentin was present. The proliferation index was low. Ancillary techniques suggested the possibility of SC in a few cytology cases; nevertheless, the final diagnosis was based on histomorphology, immunohistochemistry and genetics. The SC of salivary glands is detectable pre-operatively using ICC and genetics. The presence of the diagnostic ETV6 rearrangement increases the accuracy of FNA to the maximum. According to the Milan system, cases genetically not confirmed should be categorized as Suspicious for Malignancy or Salivary Gland Neoplasm of Uncertain Malignant Potential (SUMP), both requiring surgery.
Bioptic Laboratory Ltd Plzeň 2 Slovany Czech Republic
Department of Pathology Faculty of Medicine and Health Technology Tampere University Tampere Finland
Department of Pathology Faculty of Medicine in Plzeň Charles University Plzeň Czech Republic
Department of Pathology Fimlab Laboratories Tampere Finland
Molecular Pathology Laboratory Bioptic Laboratory Ltd Plzeň 2 Slovany Czech Republic
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