The emergence of mcr-1-mediated colistin-resistant Escherichia coli and Klebsiella pneumoniae in domestic and imported turkey meat in the Czech Republic 2017-2018
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
Grantová podpora
NV 18-09-00254
Ministerstvo Zdravotnictví Ceské Republiky
PubMed
31001764
DOI
10.1007/s12223-019-00709-z
PII: 10.1007/s12223-019-00709-z
Knihovny.cz E-zdroje
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální léková rezistence genetika MeSH
- drůbeží výrobky mikrobiologie MeSH
- Escherichia coli účinky léků genetika MeSH
- Klebsiella pneumoniae účinky léků genetika MeSH
- kolistin farmakologie MeSH
- krocani mikrobiologie MeSH
- mikrobiální testy citlivosti MeSH
- plazmidy genetika MeSH
- proteiny z Escherichia coli genetika MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- antibakteriální látky MeSH
- kolistin MeSH
- MCR-1 protein, E coli MeSH Prohlížeč
- proteiny z Escherichia coli MeSH
We investigated the occurrence of plasmid-mediated colistin resistance in Enterobacteriaceae in turkey meat produced in the Czech Republic as well as in turkey meat imported into the Czech Republic from other European Union countries. Seventeen samples of raw turkey meat from the Czech Republic (n = 4), Hungary (n = 2), Poland (n = 6) and Germany (n = 5) were cultured in peptone water at 37 °C overnight and the enriched cultures were tested for the presence of mcr-1-5 genes. PCR-positive enriched cultures were inoculated onto selective agar with colistin (3.5 mg/L). A minimal inhibitory concentration (MIC) of colistin was determined by using the broth microdilution method in PCR-positive isolates. In addition, a macrorestriction analysis was performed using XbaI endonuclease. Of 17 meat samples, 12 samples from Poland (6/6), Germany (3/5) and the Czech Republic (3/4) proved positive for the presence of the mcr-1 gene. Forty-two isolates carrying the mcr-1 gene were obtained: Escherichia coli (n = 39) revealing 32 distinct XbaI profiles and Klebsiella pneumoniae (n = 3) with 2 distinct XbaI profiles. The minimal inhibitory concentration (MIC) of the mcr-1 positive isolates was as follows: 4 mg/L (n = 28), 8 mg/L (n = 12), 32 mg/L (n = 1) and 64 mg/L (n = 1). The high prevalence (70.6%; 12/17 samples) of mcr-1-mediated colistin-resistant Enterobacteriaceae found in the turkey meat samples analysed in this study, builds on previously published evidence that poultry, and their products, represent a substantial risk for the dissemination of plasmid-mediated colistin resistance in Europe.
Zobrazit více v PubMed
PLoS One. 2016 Jul 25;11(7):e0159863 PubMed
Lancet Infect Dis. 2016 Feb;16(2):144-5 PubMed
J Antimicrob Chemother. 2016 Aug;71(8):2331-3 PubMed
Euro Surveill. 2016;21(9):30155 PubMed
Euro Surveill. 2016;21(9):30149 PubMed
Int J Antimicrob Agents. 2015 Sep;46(3):297-306 PubMed
Front Microbiol. 2018 Jun 12;9:1217 PubMed
Vet Microbiol. 2018 Jan;213:42-46 PubMed
Euro Surveill. 2016 Jul 7;21(27): PubMed
Klin Mikrobiol Infekc Lek. 2017 Jun;23(2):58-60 PubMed
Antimicrob Resist Infect Control. 2017 Aug 18;6:83 PubMed
Antimicrob Agents Chemother. 2017 Oct 24;61(11): PubMed
Lancet Infect Dis. 2016 Feb;16(2):161-8 PubMed
Int J Food Microbiol. 2017 Dec 18;263:67-73 PubMed
J Antimicrob Chemother. 2016 Aug;71(8):2338-40 PubMed
mBio. 2017 Aug 15;8(4): PubMed
J Antimicrob Chemother. 2017 Dec 1;72(12):3317-3324 PubMed
Euro Surveill. 2017 Aug 3;22(31):null PubMed
Res Vet Sci. 2016 Apr;105:134-5 PubMed
J Antimicrob Chemother. 2016 Aug;71(8):2300-5 PubMed
Euro Surveill. 2016;21(6):null PubMed
Euro Surveill. 2015;20(49):null PubMed
Front Microbiol. 2018 Aug 17;9:1880 PubMed
