A role for SPEECHLESS in the integration of leaf stomatal patterning with the growth vs disease trade-off in poplar
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
31081152
DOI
10.1111/nph.15911
Knihovny.cz E-zdroje
- Klíčová slova
- GWAS, Populus trichocarpa, amphistomaty, association genetics, immunity, stomata, trait trade-offs,
- MeSH
- alely MeSH
- celogenomová asociační studie MeSH
- druhová specificita MeSH
- fenotyp MeSH
- genotyp MeSH
- imunita rostlin genetika MeSH
- jednonukleotidový polymorfismus genetika MeSH
- kvantitativní znak dědičný MeSH
- podnebí MeSH
- Populus genetika růst a vývoj imunologie fyziologie MeSH
- průduchy rostlin genetika fyziologie MeSH
- regulace genové exprese u rostlin MeSH
- rostlinné geny MeSH
- rostlinné proteiny genetika metabolismus MeSH
- rozvržení tělního plánu * MeSH
- vývoj rostlin MeSH
- zeměpis MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- rostlinné proteiny MeSH
Occurrence of stomata on both leaf surfaces (amphistomaty) promotes higher stomatal conductance and photosynthesis while simultaneously increasing exposure to potential disease agents in black cottonwood (Populus trichocarpa). A genome-wide association study (GWAS) with 2.2M single nucleotide polymorphisms generated through whole-genome sequencing found 280 loci associated with variation in adaxial stomatal traits, implicating genes regulating stomatal development and behavior. Strikingly, numerous loci regulating plant growth and response to biotic and abiotic stresses were also identified. The most significant locus was a poplar homologue of SPEECHLESS (PtSPCH1). Individuals possessing PtSPCH1 alleles associated with greater adaxial stomatal density originated primarily from environments with shorter growing seasons (e.g. northern latitudes, high elevations) or with less precipitation. PtSPCH1 was expressed in developing leaves but not developing stem xylem. In developing leaves, RNA sequencing showed patterns of coordinated expression between PtSPCH1 and other GWAS-identified genes. The breadth of our GWAS results suggests that the evolution of amphistomaty is part of a larger, complex response in plants. Suites of genes underpin this response, retrieved through genetic association to adaxial stomata, and show coordinated expression during development. We propose that the occurrence of amphistomaty in P. trichocarpa involves PtSPCH1 and reflects selection for supporting rapid growth over investment in immunity.
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