Essential Methods of Plant Sample Preparation for High-Resolution Scanning Electron Microscopy at Room Temperature
Methods in molecular biology (Clifton, N.J.).
2019 ;
1992 () :
63-76.
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
Perzistentní odkaz
https://www.medvik.cz/link/pmid31148031
- Klíčová slova
- Chemical fixation, Critical point drying, Dehydration, High-resolution scanning electron microscope, Metal coating, Plant sample, Sample storage, t-Butanol freeze drying,
- MeSH
- fixace tkání metody MeSH
- lyofilizace metody MeSH
- mikroskopie elektronová rastrovací metody MeSH
- rostliny ultrastruktura MeSH
- teplota MeSH
- vysoušení metody MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
In this chapter, conventional techniques are described for the preparation of plant samples at room temperature before examination in the high resolution scanning electron microscopy. Protocols are given on how to collect, to fix, to dehydrate, and to dry plant samples. Subsequently, it is described how to stick them to stubs and cover with a thin conductive layer. These methods are suitable for a wide variety of plant specimens, ranging from microalgae to higher plants.
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