Antisense Oligodeoxynucleotide-Mediated Gene Knockdown in Pollen Tubes
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- Klíčová slova
- Antisense oligodeoxynucleotide, Gene knockdown, Pollen tube, Tip growth,
- MeSH
- antisense oligodeoxyribonukleotidy genetika MeSH
- genový knockdown metody MeSH
- pylová láčka genetika MeSH
- regulace genové exprese u rostlin MeSH
- tabák genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antisense oligodeoxyribonukleotidy MeSH
Specific gene knockdown mediated by the antisense oligodeoxynucleotides (AODNs) strategy emerged as a rapid and effective tool for probing gene role in plant cells, particularly tip-growing pollen tubes. Here, we describe the protocol for the successful employment of AODN technique in growing tobacco pollen tubes, covering AODN design, application, and analysis of the results. We also discuss the advantages and drawbacks of this method.
Department of Experimental Plant Biology Faculty of Science Charles University Prague Czech Republic
Institute of Experimental Botany Czech Academy of Sciences Prague Czech Republic
Citace poskytuje Crossref.org
Regulation of Exocyst Function in Pollen Tube Growth by Phosphorylation of Exocyst Subunit EXO70C2
