Antisense Oligodeoxynucleotide-Mediated Gene Knockdown in Pollen Tubes
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Antisense oligodeoxynucleotide, Gene knockdown, Pollen tube, Tip growth,
- MeSH
- Oligodeoxyribonucleotides, Antisense genetics MeSH
- Gene Knockdown Techniques methods MeSH
- Pollen Tube genetics MeSH
- Gene Expression Regulation, Plant MeSH
- Nicotiana genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Oligodeoxyribonucleotides, Antisense MeSH
Specific gene knockdown mediated by the antisense oligodeoxynucleotides (AODNs) strategy emerged as a rapid and effective tool for probing gene role in plant cells, particularly tip-growing pollen tubes. Here, we describe the protocol for the successful employment of AODN technique in growing tobacco pollen tubes, covering AODN design, application, and analysis of the results. We also discuss the advantages and drawbacks of this method.
Department of Experimental Plant Biology Faculty of Science Charles University Prague Czech Republic
Institute of Experimental Botany Czech Academy of Sciences Prague Czech Republic
References provided by Crossref.org
Regulation of Exocyst Function in Pollen Tube Growth by Phosphorylation of Exocyst Subunit EXO70C2
