Tubes for detection of cholinesterase inhibitors-Unique effects of Neusilin on the stability of butyrylcholinesterase-impregnated carriers
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
31186107
DOI
10.1016/j.enzmictec.2019.05.002
PII: S0141-0229(19)30069-9
Knihovny.cz E-resources
- Keywords
- Cholinesterase, Detection, Enzyme, Nerve agent, Neusilin, Stability,
- MeSH
- Biosensing Techniques methods MeSH
- Butyrylcholinesterase chemistry metabolism MeSH
- Cholinesterase Inhibitors analysis MeSH
- Enzymes, Immobilized chemistry metabolism MeSH
- Colorimetry methods MeSH
- Drug Carriers chemistry MeSH
- Silicates metabolism MeSH
- Aluminum Compounds metabolism MeSH
- Magnesium Compounds metabolism MeSH
- Enzyme Stability drug effects MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- aluminum magnesium silicate MeSH Browser
- Butyrylcholinesterase MeSH
- Cholinesterase Inhibitors MeSH
- Enzymes, Immobilized MeSH
- Drug Carriers MeSH
- Silicates MeSH
- Aluminum Compounds MeSH
- Magnesium Compounds MeSH
Detection tubes are small devices for the colorimetric enzymatic detection of cholinesterase inhibitors such as sarin, soman, VX nerve agents and substances denoted as Novichok. These detectors contain carriers in the form of pellets with immobilized cholinesterase, substrate and detection reagent. Their advantages are portability, sensitivity and simplicity, enabling fast detection of such compounds from air and water in case of a terrorist attack or war. In general, maintaining the stability of an enzyme for a longer time is very problematic; therefore, its further enhancement is required for safety and financial reasons. In this study, the stability of our patented carriers in the form of pellets with immobilized butyrylcholinesterase containing an increasing amount of the unique sorbent Neusilin® US2 was evaluated. The samples containing Neusilin maintained the stability of the immobilized enzyme for a longer time even at higher temperature and humidity than the currently commercially used carrier without Neusilin, allowing improved detection of nerve agents.
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