N-acetylcysteine dual and antagonistic effect on cadmium cytotoxicity in human leukemia cells
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
31288199
DOI
10.1016/j.etap.2019.103213
PII: S1382-6689(19)30084-5
Knihovny.cz E-zdroje
- Klíčová slova
- Cadmium chelation, Cadmium cytotoxicity, K562 cells, N-acetylcysteine, Reactive oxygen species, U937 cells,
- MeSH
- acetylcystein farmakologie MeSH
- buňky K562 MeSH
- chelátory farmakologie MeSH
- kadmium toxicita MeSH
- látky znečišťující životní prostředí toxicita MeSH
- lidé MeSH
- reaktivní formy kyslíku metabolismus MeSH
- U937 buňky MeSH
- viabilita buněk účinky léků MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- acetylcystein MeSH
- chelátory MeSH
- kadmium MeSH
- látky znečišťující životní prostředí MeSH
- reaktivní formy kyslíku MeSH
Although cadmium (Cd2+) is unable to form reactive oxygen species (ROS) directly, many of its adverse effects are connected to increased ROS generation resulting in cell death. In support of this supposition, a large number of studies have shown protective effects of antioxidants such as N-acetylcysteine (NAC) against cadmium induced cytotoxicity. Here, we describe the cytotoxic effects of Cd2+ on human leukemia U937 and K562 cells that were not mediated by oxidative stress. Surprisingly, we observed that addition of low concentrations of NAC can drastically potentiate cadmium cytotoxicity solely via ROS production. However, all adverse effects of the metal were prevented by NAC at high concentrations. Detailed analysis indicated that the protective effect of NAC was mediated by its ability to form stable complex with cadmium [Cd(NAC)2]. In conclusion, NAC exhibits dual and antagonistic effects on Cd2+ cytotoxicity in human leukemia cells.
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