Here, we describe two plasmids carrying mcr-4.3 in two Acinetobacter baumannii strains isolated from imported food and a clinical sample. The comparative analysis of these plasmids, with two other plasmids reported in the NCBI database, highlighted the common origin of the plasmidic structure carrying mcr-4.3 This is the first case of the mcr-4.3 gene in a A. baumannii strain isolated from a clinical case in Europe. We hypothesize that food import is initiating the spread in Czech Republic.

CEITEC VFU University of Veterinary and Pharmaceutical Sciences Brno Brno Czech Republic

Department of Biology and Wildlife Diseases Faculty of Veterinary Hygiene and Ecology University of Veterinary and Pharmaceutical Sciences Brno Brno Czech Republic

Department of Clinical Microbiology Faculty of Medicine and University Hospital Charles University Hradec Kralove Czech Republic

Faculty of Medicine Biomedical Center Charles University Plzen Czech Republic

National Reference Laboratory for Antibiotics The National Institute of Public Health Centre for Epidemiology and Microbiology Prague Czech Republic

Veterinary Research Institute Brno Czech Republic

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