Complete Nucleotide Sequences of mcr-4.3-Carrying Plasmids in Acinetobacter baumannii Sequence Type 345 of Human and Food Origin from the Czech Republic, the First Case in Europe
Language English Country United States Media electronic-print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
31332072
PubMed Central
PMC6761559
DOI
10.1128/aac.01166-19
PII: AAC.01166-19
Knihovny.cz E-resources
- Keywords
- Acinetobacter baumannii, WGS, colistin resistance, mcr-4.3,
- MeSH
- Acinetobacter baumannii genetics MeSH
- Drug Resistance, Bacterial genetics MeSH
- Humans MeSH
- Microbial Sensitivity Tests MeSH
- Peptides genetics MeSH
- Plasmids genetics MeSH
- Aged, 80 and over MeSH
- Check Tag
- Humans MeSH
- Aged, 80 and over MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Europe MeSH
- Names of Substances
- Peptides MeSH
Here, we describe two plasmids carrying mcr-4.3 in two Acinetobacter baumannii strains isolated from imported food and a clinical sample. The comparative analysis of these plasmids, with two other plasmids reported in the NCBI database, highlighted the common origin of the plasmidic structure carrying mcr-4.3 This is the first case of the mcr-4.3 gene in a A. baumannii strain isolated from a clinical case in Europe. We hypothesize that food import is initiating the spread in Czech Republic.
CEITEC VFU University of Veterinary and Pharmaceutical Sciences Brno Brno Czech Republic
Faculty of Medicine Biomedical Center Charles University Plzen Czech Republic
See more in PubMed
Carattoli A, Villa L, Feudi C, Curcio L, Orsini S, Luppi A, Pezzotti G, Magistrali CF. 2017. Novel plasmid-mediated colistin resistance mcr-4 gene in Salmonella and Escherichia coli, Italy 2013, Spain and Belgium, 2015 to 2016. Euro Surveill 22:30589. doi:10.2807/1560-7917.ES.2017.22.31.30589. PubMed DOI PMC
Partridge SR, Di Pilato V, Doi Y, Feldgarden M, Haft DH, Klimke W, Kumar-Singh S, Liu J-H, Malhotra-Kumar S, Prasad A, Rossolini GM, Schwarz S, Shen J, Walsh T, Wang Y, Xavier BB. 2018. Proposal for assignment of allele numbers for mobile colistin resistance (mcr) genes. J Antimicrob Chemother 73:2625–2630. doi:10.1093/jac/dky262. PubMed DOI PMC
Teo JWP, Kalisvar M, Venkatachalam I, Ng OT, Lin RTP, Octavia S. 2017. mcr-3 and mcr-4 variants in carbapenemase-producing clinical Enterobacteriaceae do not confer phenotypic polymyxin resistance. J Clin Microbiol 56:e01562-17. doi:10.1128/JCM.01562-17. PubMed DOI PMC
Pagano M, Poirel L, Martins AF, Rozales FP, Zavascki AP, Barth AL, Nordmann P. 2015. Emergence of NDM-1-producing Acinetobacter pittii in Brazil. Int J Antimicrob Agents 45:444–445. doi:10.1016/j.ijantimicag.2014.12.011. PubMed DOI
Zankari E, Hasman H, Cosentino S, Vestergaard M, Rasmussen S, Lund O, Aarestrup FM, Larsen MV. 2012. Identification of acquired antimicrobial resistance genes. J Antimicrob Chemother 67:2640–2644. doi:10.1093/jac/dks261. PubMed DOI PMC
Langmead B, Salzberg SL. 2012. Fast gapped-read alignment with Bowtie 2. Nat Methods 9:357–359. doi:10.1038/nmeth.1923. PubMed DOI PMC
Koboldt DC, Zhang Q, Larson DE, Shen D, Mclellan MD, Lin L, Miller CA, Mardis ER, Ding L, Wilson RK. 2012. VarScan 2: somatic mutation and copy number alteration discovery in cancer by exome sequencing. Genome Res 22:568–576. doi:10.1101/gr.129684.111. PubMed DOI PMC
Milne I, Stephen G, Bayer M, Cock PJA, Pritchard L, Cardle L, Shaw PD, Marshall D. 2013. Using Tablet for visual exploration of second-generation sequencing data. Brief Bioinform 14:193–202. doi:10.1093/bib/bbs012. PubMed DOI
Seemann T. 2014. Prokka: rapid prokaryotic genome annotation. Bioinformatics 30:2068–2069. doi:10.1093/bioinformatics/btu153. PubMed DOI
Gurevich A, Saveliev V, Vyahhi N, Tesler G. 2013. QUAST: quality assessment tool for genome assemblies. Bioinformatics 29:1072–1075. doi:10.1093/bioinformatics/btt086. PubMed DOI PMC
Oikonomou O, Sarrou S, Papagiannitsis CC, Georgiadou S, Mantzarlis K, Zakynthinos E, Dalekos GN, Petinaki E. 2015. Rapid dissemination of colistin and carbapenem resistant Acinetobacter baumannii in Central Greece: mechanisms of resistance, molecular identification and epidemiological data. BMC Infect Dis 15:559. doi:10.1186/s12879-015-1297-x. PubMed DOI PMC
Tsuchiya Y, Zhyvoloup A, Baković J, Thomas N, Yu BYK, Das S, Orengo C, Newell C, Ward J, Saladino G, Comitani F, Gervasio FL, Malanchuk OM, Khoruzhenko AI, Filonenko V, Peak-Chew SY, Skehel M, Gout I. 2018. Protein CoAlation and antioxidant function of coenzyme A in prokaryotic cells. Biochem J 475:1909–1937. doi:10.1042/BCJ20180043. PubMed DOI PMC
Ma F, Shen C, Zheng X, Liu Y, Chen H, Zhong L, Liang Y, Liao K, Xia Y, Tian G-B, Yang Y. 2019. Identification of a novel plasmid carrying mcr-4.3 in an Acinetobacter baumannii strain in China. Antimicrob Agents Chemother 63:e00133-19. doi:10.1128/AAC.00133-19. PubMed DOI PMC
Detection of Five mcr-9-Carrying Enterobacterales Isolates in Four Czech Hospitals
