Multifunctional polymeric implant coatings based on gelatin, hyaluronic acid derivative and chain length-controlled poly(arginine)
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
31499960
DOI
10.1016/j.msec.2019.109898
PII: S0928-4931(19)31187-7
Knihovny.cz E-resources
- Keywords
- Antimicrobial coatings, ECM, Implants, Multifunctionality,
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Coated Materials, Biocompatible pharmacology MeSH
- Human Umbilical Vein Endothelial Cells drug effects MeSH
- Extracellular Matrix metabolism MeSH
- Hyaluronic Acid pharmacology MeSH
- Humans MeSH
- Peptides pharmacology MeSH
- Polymers pharmacology MeSH
- Prostheses and Implants * MeSH
- Cattle MeSH
- Vascular Endothelial Growth Factor A metabolism MeSH
- Gelatin pharmacology MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- Coated Materials, Biocompatible MeSH
- Hyaluronic Acid MeSH
- Peptides MeSH
- polyarginine MeSH Browser
- Polymers MeSH
- Vascular Endothelial Growth Factor A MeSH
- Gelatin MeSH
Surface of the implantable devices is the root cause of several complications such as infections, implant loosening and chronic inflammation. There is an urgent need for multifunctional coatings that can address these shortcomings simultaneously in a manner similar to the structures of extracellular matrix. Herein, we developed a coating system composed of ECM components and a naturally derived polypeptide. The interactions between the coating components create an environment that enables incorporation of an antimicrobial/angiogenic polypeptide. The film composition is based gelatin and hyaluronic acid modified with aldehyde groups (HA-Ald) that can react with poly (arginine) (PAR) through transient interactions. Nanoplasmon measurements demonstrated a significantly higher loading of PAR in films containing HA-Ald with longer retention of PAR in the structure. The presence of PAR not only provides to the film surface antimicrobial (contact-killing) properties but also increased endothelial cell-cell contacts (PECAM) and VEGFA gene expression and secretion by human vascular endothelial cells. This multifunctional coating can be easily applied to surface of implants where it can enact on several problems simultaneously.
Contipro Biotech S R O Dolni Dobrouc 401 561 02 Dolni Dobrouc Czech Republic
FAB Université Lyon 1 CNRS 5246 ICBMS Lyon France
INSERM UMR 1121 11 rue Humann 67085 Strasbourg France
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