The effect of exposure to hypoxia on superoxide formation by alveolar macrophages is indirect
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
31518607
DOI
10.1016/j.lfs.2019.116864
PII: S0024-3205(19)30791-X
Knihovny.cz E-zdroje
- Klíčová slova
- Alveolar macrophage, Chronic hypoxia, Macrophage polarization, Superoxide,
- MeSH
- alveolární makrofágy metabolismus patologie MeSH
- bronchoalveolární lavážní tekutina chemie MeSH
- hypoxie patofyziologie MeSH
- krysa rodu Rattus MeSH
- kultivované buňky MeSH
- potkani Wistar MeSH
- superoxidy metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- superoxidy MeSH
AIMS: To elucidate the role of alveolar macrophages (AM) in the pathogenesis of hypoxic pulmonary hypertension (HPH), we tested the effects of sustained hypoxia on AM polarization and on the formation of superoxide by AM in vivo and in vitro. MAIN METHODS: Rat AM were obtained by bronchoalveolar lavage. 4-day exposure to hypoxia (10% O2) was carried out in vivo (rats in isobaric hypoxic chamber, controls kept in air) or in vitro (control AM in 21% O2 and 5% CO2). Superoxide production was measured by luminol-orthovanadate chemiluminescence, AM polarization was detected immunocytochemically. To ascertain the effect of substances contained in the alveolar environment, we cultivated cells also in the presence of non-cellular components of the bronchoalveolar lavage fluid (BALF) either from controls or from rats exposed to 4 days of hypoxia. KEY FINDINGS: In vivo, but not in vitro, hypoxia increased AM superoxide production. Both types of hypoxia polarized AM into M2 (pro-proliferative) type. While the presence of control BALF attenuated superoxide production in AM cultivated in normoxia, BALF from the hypoxia-exposed rats had no effect. In AM cultivated in hypoxia, superoxide production was not altered by control BALF and elevated by BALF obtained from hypoxic rats. SIGNIFICANCE: Hypoxia does not influence superoxide production by AM directly but rather by modulating their milieu and their sensitivity to external influences.
Department of Histology and Embryology 2nd Faculty of Medicine Charles University Czech Republic
Department of Pathophysiology 2nd Faculty of Medicine Charles University Czech Republic
Department of Physiology 2nd Faculty of Medicine Charles University Czech Republic
Citace poskytuje Crossref.org
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