In the current study, we present methods of sodium hyaluronate, also denoted as hyaluronan (HA), nanofiber fabrication using a direct-current (DC) electric field. HA was spun in combination with poly(vinyl alcohol) (PVA) and polyethylene oxide (PEO) and as a pure polymer. Nonaggressive solvents were used due to the possible use of the fibers in life sciences. The influences of polymer concentration, average molecular weight (Mw), viscosity, and solution surface tension were analyzed. HA and PVA were fluorescent-labeled in order to examine the electrospun structures using fluorescence confocal microscopy. In this study, two intermediate solvent mixtures that facilitate HA electrospinning were found. In the case of polymer co-electrospinning, the effect of the surfactant content on the HA/PVA electrospinning process, and the effect of HA Mw on HA/PEO nanofiber morphology, were examined, respectively.

CEBIA Tech Faculty of Applied Informatics Thomas Bata University in Zlín Nad Stráněmi 4511 760 05 Zlín Czech Republic

Center of Polymer Systems Thomas Bata University in Zlín tř Tomáše Bati 5678 760 01 Zlín Czech Republic

Department of Physics and Materials Engineering Faculty of Technology Thomas Bata University in Zlín Vavrečkova 275 760 01 Zlín Czech Republic

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