The article describes preparation, characterization and further modification of hybrid magnetic particles (Au nanoshells with a magnetic core (MPs@silica@Au)) by zwitterionic molecules bearing diazonium functional groups. Such hybrid magnetic particles modified by zwitterionic molecules exhibit the following features: •Responsiveness towards external magnetic field applicable for various enrichment strategies due to magnetic core;•Golden outer layer exhibiting free surface plasmons could be used for grafting of zwitterionic molecules via diazonium functionality;•Zwitterionic interface on such particles provides resistivity towards non-specific protein binding; and at the same time such interface was applied for immobilization of antibodies against prostate specific antigen (PSA) applied for selective enrichment of PSA from serum samples with subsequent electrochemical assays. The approach presented here using hybrid magnetic particles can be easily applied for immobilization of antibodies using a highly robust surface patterning protocols i.e. by formation of a self-assembled monolayer with delivery of functional groups on the outer surface of magnetic particles. Hybrid magnetic particles with immobilized antibodies are applied for highly efficient and quick separation of protein of interest i.e. PSA from complex sample. Finally, hybrid magnetic particles with "fished-out" protein molecules could be incubated with lectins to form a sandwich configuration for glycoprofiling of PSA.

Center for Advanced Materials Qatar University P O Box 2713 Doha Qatar

Glycanostics Ltd Dubravska cesta 9 Bratislava 845 38 Slovak Republic

Institute of Chemistry Slovak Academy of Sciences Dubravska cesta 9 Bratislava 845 38 Slovak Republic

Institute of Physics of the Czech Academy of Sciences Cukrovarnicka 10 112 Prague 162 00 Czech Republic

Polymer Institute Slovak Academy of Sciences Dubravska cesta 9 Bratislava 845 41 Slovak Republic

Private Urological Ambulance Piaristicka 6 Trencin 911 01 Slovak Republic

St Elisabeth Cancer Institute Heydukova 10 Bratislava 812 50 Slovak Republic

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Validating fPSA Glycoprofile as a Prostate Cancer Biomarker to Avoid Unnecessary Biopsies and Re-Biopsies

Advanced impedimetric biosensor configuration and assay protocol for glycoprofiling of a prostate oncomarker using Au nanoshells with a magnetic core