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PubMed

Záznam pochází z PubMed

Development of a nanomedicine-loaded hydrogel for sustained delivery of an angiogenic growth factor to the ischaemic myocardium

O'Dwyer, Joanne
Autor O'Dwyer, Joanne Drug Delivery & Advanced Materials Team, School of Pharmacy & Biomolecular Sciences, Royal College of Surgeons in Ireland (RCSI), Dublin 2, Ireland Tissue Engineering Research Group, Department of Anatomy & Regenerative Medicine, RCSI, 1st Floor, Ardilaun House (Block B), St. Stephen's Green, Dublin 2, Ireland Trinity Centre for Biomedical Engineering, Trinity College Dublin (TCD), Dublin 2, Ireland
Murphy, Robert
Autor Murphy, Robert Department of Chemistry, RCSI, Dublin 2, Ireland
Dolan, Eimear B
Autor Dolan, Eimear B Drug Delivery & Advanced Materials Team, School of Pharmacy & Biomolecular Sciences, Royal College of Surgeons in Ireland (RCSI), Dublin 2, Ireland Tissue Engineering Research Group, Department of Anatomy & Regenerative Medicine, RCSI, 1st Floor, Ardilaun House (Block B), St. Stephen's Green, Dublin 2, Ireland
Kovarova, Lenka
Autor Kovarova, Lenka R&D Department, Contipro, Dolni Dobrouc 401, 561 02, Dolni Dobrouc, Czech Republic Faculty of Chemistry, Institute of Physical Chemistry, Brno University of Technology, Purkynova 464/118, 612 00, Brno, Czech Republic
Pravda, Martin
Autor Pravda, Martin R&D Department, Contipro, Dolni Dobrouc 401, 561 02, Dolni Dobrouc, Czech Republic
Velebny, Vladimir
Autor Velebny, Vladimir R&D Department, Contipro, Dolni Dobrouc 401, 561 02, Dolni Dobrouc, Czech Republic
Heise, Andreas
Autor Heise, Andreas Department of Chemistry, RCSI, Dublin 2, Ireland CÚRAM, SFI Research Centre for Medical Devices, National University of Ireland Galway (NUIG) & RCSI, Galway, Ireland AMBER, the SFI Centre for Advanced Materials and Bioengineering, NUIG, RCSI & TCD, Dublin, Ireland
Duffy, Garry P
Autor Duffy, Garry P Tissue Engineering Research Group, Department of Anatomy & Regenerative Medicine, RCSI, 1st Floor, Ardilaun House (Block B), St. Stephen's Green, Dublin 2, Ireland Trinity Centre for Biomedical Engineering, Trinity College Dublin (TCD), Dublin 2, Ireland CÚRAM, SFI Research Centre for Medical Devices, National University of Ireland Galway (NUIG) & RCSI, Galway, Ireland AMBER, the SFI Centre for Advanced Materials and Bioengineering, NUIG, RCSI & TCD, Dublin, Ireland Anatomy, School of Medicine, College of Medicine, Nursing and Health Sciences, NUIG, Galway, Ireland
Cryan, Sally Ann
Autor Cryan, Sally Ann ORCID Drug Delivery & Advanced Materials Team, School of Pharmacy & Biomolecular Sciences, Royal College of Surgeons in Ireland (RCSI), Dublin 2, Ireland. scryan@rcsi.ie Tissue Engineering Research Group, Department of Anatomy & Regenerative Medicine, RCSI, 1st Floor, Ardilaun House (Block B), St. Stephen's Green, Dublin 2, Ireland. scryan@rcsi.ie Trinity Centre for Biomedical Engineering, Trinity College Dublin (TCD), Dublin 2, Ireland. scryan@rcsi.ie CÚRAM, SFI Research Centre for Medical Devices, National University of Ireland Galway (NUIG) & RCSI, Galway, Ireland. scryan@rcsi.ie AMBER, the SFI Centre for Advanced Materials and Bioengineering, NUIG, RCSI & TCD, Dublin, Ireland. scryan@rcsi.ie

Drug delivery and translational research. 2020 Apr ; 10 (2) : 440-454.

Drug Deliv Transl Res
ISSN 2190-3948 | 2190-393X
Zdroj

Jazyk angličtina Země Spojené státy americké Médium print

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz https://www.medvik.cz/link/pmid31691161

PubMed 31691161
DOI 10.1007/s13346-019-00684-5
PII: 10.1007/s13346-019-00684-5
Knihovny.cz E-zdroje

Klíčová slova
Angiogenesis, Growth factor, Ischaemia, Nanoparticle, Star polypeptide,
MeSH
aplikace kožní MeSH
endoteliální buňky pupečníkové žíly (lidské) MeSH
hydrogely MeSH
ischemická choroba srdeční farmakoterapie MeSH
kyselina polyglutamová chemie MeSH
léky s prodlouženým účinkem MeSH
lidé MeSH
nanočástice MeSH
statická elektřina MeSH
vaskulární endoteliální růstový faktor A chemie farmakologie MeSH
Check Tag
lidé MeSH
Publikační typ
časopisecké články MeSH
práce podpořená grantem MeSH
Názvy látek
hydrogely MeSH
kyselina polyglutamová MeSH
léky s prodlouženým účinkem MeSH
vaskulární endoteliální růstový faktor A MeSH

The 5-year mortality rate for heart failure borders on 50%. The main cause is an ischaemic cardiac event where blood supply to the tissue is lost and cell death occurs. Over time, this damage spreads and the heart is no longer able to pump efficiently. Increasing vascularisation of the affected area has been shown to reduce patient symptoms. The growth factors required to do this have short half-lives making development of an efficacious therapy difficult. Herein, the angiogenic growth factor Vascular Endothelial Growth Factor (VEGF) is complexed electrostatically with star-shaped or linear polyglutamic acid (PGA) polypeptides. Optimised PGA-VEGF nanomedicines provide VEGF encapsulation of > 99% and facilitate sustained release of VEGF for up to 28 days in vitro. The star-PGA-VEGF nanomedicines are loaded into a percutaneous delivery compliant hyaluronic acid hydrogel. Sustained release of VEGF from the composite nano-in-gel system is evident for up to 35 days and the released VEGF has comparable bioactivity to free, fresh VEGF when tested on both Matrigel® and scratch assays. The final star-PGA-VEGF nanomedicine-loaded hydrogel is biocompatible and provides sustained release of bioactive VEGF. Therefore, we report the development of novel, self-assembling PGA-VEGF nanomedicines and their incorporation into a hyaluronic acid hydrogel that is compatible with medical devices to enable minimally invasive delivery to the heart. The final star-PGA-VEGF nanomedicine-loaded hydrogel is biocompatible and provides sustained release of bioactive VEGF. This formulation provides the basis for optimal spatiotemporal delivery of an angiogenic growth factor to the ischaemic myocardium.

AMBER the SFI Centre for Advanced Materials and Bioengineering NUIG RCSI and TCD Dublin Ireland

Anatomy School of Medicine College of Medicine Nursing and Health Sciences NUIG Galway Ireland

CÚRAM SFI Research Centre for Medical Devices National University of Ireland Galway and RCSI Galway Ireland

Department of Chemistry RCSI Dublin 2 Ireland

Drug Delivery and Advanced Materials Team School of Pharmacy and Biomolecular Sciences Royal College of Surgeons in Ireland Dublin 2 Ireland

Faculty of Chemistry Institute of Physical Chemistry Brno University of Technology Purkynova 464 118 612 00 Brno Czech Republic

R and D Department Contipro Dolni Dobrouc 401 561 02 Dolni Dobrouc Czech Republic

Tissue Engineering Research Group Department of Anatomy and Regenerative Medicine RCSI 1st Floor Ardilaun House St Stephen's Green Dublin 2 Ireland

Trinity Centre for Biomedical Engineering Trinity College Dublin Dublin 2 Ireland

Zobrazit více v PubMed

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Biomaterials. 2010 Feb;31(6):1235-41 PubMed