Screening of Beta-Secretase Inhibitors by Capillary Electrophoresis-Mass Spectrometry
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- Klíčová slova
- Alzheimer’s disease, Beta-secretase, Capillary electrophoresis, Enzyme inhibition, Mass spectrometry, Screening,
- MeSH
- Alzheimerova nemoc farmakoterapie metabolismus MeSH
- amyloidový prekurzorový protein beta metabolismus MeSH
- aspartátové endopeptidasy metabolismus MeSH
- elektroforéza kapilární metody MeSH
- hmotnostní spektrometrie metody MeSH
- inhibitory enzymů farmakologie MeSH
- inhibitory proteas farmakologie MeSH
- kinetika MeSH
- lidé MeSH
- plošný screening metody MeSH
- sekretasy antagonisté a inhibitory MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- amyloidový prekurzorový protein beta MeSH
- aspartátové endopeptidasy MeSH
- inhibitory enzymů MeSH
- inhibitory proteas MeSH
- sekretasy MeSH
Alzheimer's disease is the most common cause of dementia, currently afflicting almost 40 million patients worldwide. According to the amyloid cascade hypothesis, the pathogenesis of the disease could be slowed down or even stopped by the inhibition of beta-secretase, making this aspartic acid protease a potentially important drug target site. Capillary electrophoresis is a promising technique for screening putative enzyme inhibitors due to highly effective separations, minuscule sample and other chemicals consumption, compatibility with a variety of detection techniques, and high throughput via automation. This chapter presents a method based on capillary electrophoresis coupled to mass spectrometry detection for kinetic and inhibition assays of the beta-secretase reaction with a decapeptide derived from an amyloid precursor protein.
Citace poskytuje Crossref.org
