Interleukin-10 production by B cells is regulated by cytokines, but independently of GATA-3 or FoxP3 expression
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
31787200
DOI
10.1016/j.cellimm.2019.103987
PII: S0008-8749(19)30189-3
Knihovny.cz E-zdroje
- Klíčová slova
- B cell, Cytokine, FoxP3, GATA-3, IL-10 production, T cell,
- MeSH
- aktivace lymfocytů imunologie MeSH
- faktor 1 indukovatelný hypoxií - podjednotka alfa imunologie MeSH
- forkhead transkripční faktory metabolismus MeSH
- interferon gama imunologie MeSH
- interleukin-10 biosyntéza MeSH
- interleukin-4 imunologie MeSH
- kultivované buňky MeSH
- lipopolysacharidy imunologie MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- regulační B-lymfocyty imunologie MeSH
- regulační T-lymfocyty imunologie MeSH
- Th2 buňky imunologie MeSH
- transformující růstový faktor beta imunologie MeSH
- transkripční faktor GATA3 metabolismus MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- faktor 1 indukovatelný hypoxií - podjednotka alfa MeSH
- forkhead transkripční faktory MeSH
- Foxp3 protein, mouse MeSH Prohlížeč
- Gata3 protein, mouse MeSH Prohlížeč
- Hif1a protein, mouse MeSH Prohlížeč
- IFNG protein, mouse MeSH Prohlížeč
- IL10 protein, mouse MeSH Prohlížeč
- Il4 protein, mouse MeSH Prohlížeč
- interferon gama MeSH
- interleukin-10 MeSH
- interleukin-4 MeSH
- lipopolysacharidy MeSH
- transformující růstový faktor beta MeSH
- transkripční faktor GATA3 MeSH
The knowledge of mechanisms of regulation of IL-10 production by B cells remains still very limited. We show here that highly purified mouse B cells stimulated with LPS produce significant levels of IL-10, but Bregs in our model do not express detectable level of either Foxp3 or GATA-3. Nevertheless, IL-10 production by B cells is regulated by cytokines. In activated B cells, IL-10 production was significantly enhanced by IFN-γ and decreased in the presence of IL-4 or TGF-β. These findings are in sharp contrast with the observations in T cells, where IL-10 production correlates with GATA-3 or FoxP3 expression, and the cytokines regulate IL-10 production in a reverse manner than in activated B cells. These results thus show that the production of IL-10 by Bregs is regulated by cytokines independently of the expression of GATA-3 and FoxP3, which is clearly different from GATA-3-dependent IL-10 production by activated Th2 cells and FoxP3 expression in IL-10-producing Tregs.
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