Interleukin-10 production by B cells is regulated by cytokines, but independently of GATA-3 or FoxP3 expression
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
31787200
DOI
10.1016/j.cellimm.2019.103987
PII: S0008-8749(19)30189-3
Knihovny.cz E-resources
- Keywords
- B cell, Cytokine, FoxP3, GATA-3, IL-10 production, T cell,
- MeSH
- Lymphocyte Activation immunology MeSH
- Hypoxia-Inducible Factor 1, alpha Subunit immunology MeSH
- Forkhead Transcription Factors metabolism MeSH
- Interferon-gamma immunology MeSH
- Interleukin-10 biosynthesis MeSH
- Interleukin-4 immunology MeSH
- Cells, Cultured MeSH
- Lipopolysaccharides immunology MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- B-Lymphocytes, Regulatory immunology MeSH
- T-Lymphocytes, Regulatory immunology MeSH
- Th2 Cells immunology MeSH
- Transforming Growth Factor beta immunology MeSH
- GATA3 Transcription Factor metabolism MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Hypoxia-Inducible Factor 1, alpha Subunit MeSH
- Forkhead Transcription Factors MeSH
- Foxp3 protein, mouse MeSH Browser
- Gata3 protein, mouse MeSH Browser
- Hif1a protein, mouse MeSH Browser
- IFNG protein, mouse MeSH Browser
- IL10 protein, mouse MeSH Browser
- Il4 protein, mouse MeSH Browser
- Interferon-gamma MeSH
- Interleukin-10 MeSH
- Interleukin-4 MeSH
- Lipopolysaccharides MeSH
- Transforming Growth Factor beta MeSH
- GATA3 Transcription Factor MeSH
The knowledge of mechanisms of regulation of IL-10 production by B cells remains still very limited. We show here that highly purified mouse B cells stimulated with LPS produce significant levels of IL-10, but Bregs in our model do not express detectable level of either Foxp3 or GATA-3. Nevertheless, IL-10 production by B cells is regulated by cytokines. In activated B cells, IL-10 production was significantly enhanced by IFN-γ and decreased in the presence of IL-4 or TGF-β. These findings are in sharp contrast with the observations in T cells, where IL-10 production correlates with GATA-3 or FoxP3 expression, and the cytokines regulate IL-10 production in a reverse manner than in activated B cells. These results thus show that the production of IL-10 by Bregs is regulated by cytokines independently of the expression of GATA-3 and FoxP3, which is clearly different from GATA-3-dependent IL-10 production by activated Th2 cells and FoxP3 expression in IL-10-producing Tregs.
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