Pythium oligandrum is a soil born free living oomycete able to parasitize fungi and oomycetes prey, including important plant and animals pathogens. Pythium oligandrum can colonize endophytically the root tissues of diverse plants where it induces plant defenses. Here we report the first long-read genome sequencing of a P. oligandrum strain sequenced by PacBio technology. Sequencing of genomic DNA loaded onto six SMRT cells permitted the acquisition of 913,728 total reads resulting in 112X genome coverage. The assembly and polishing of the genome sequence yielded180 contigs (N50 = 1.3 Mb; L50 = 12). The size of the genome assembly is 41.9 Mb with a longest contig of 2.7 Mb and 15,007 predicted protein-coding genes among which 95.25% were supported by RNAseq data, thus constituting a new Pythium genome reference. This data will facilitate genomic comparisons of Pythium species that are commensal, beneficial or pathogenic on plant, or parasitic on fungi and oomycete to identify key genetic determinants underpinning their diverse lifestyles. In addition comparison with plant pathogenic or zoopathogenic species will illuminate genomic adaptations for pathogenesis toward widely diverse hosts.

Biopreparáty spol s r o Tylišovská 772 1 16000 Praha 6 Czech Republic and

CNRS Fédération de Recherches Agrobiosciences Interactions Biodiversity Plateforme d'Imagerie TRI Castanet Tolosan France

De Sangosse Bonnel 47480 Pont Du Casse France

INRA US 1426 GeT PlaGe Genotoul Castanet Tolosan France

Laboratoire de Recherche en Sciences Végétales Université de Toulouse CNRS UPS 24 Chemin de Borde Rouge Auzeville BP42617 31326 Castanet Tolosan France

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Novel Insights into the Effect of Pythium Strains on Rapeseed Metabolism