Pythium oligandrum Dotaz Zobrazit nápovědu
Tato kazuistika popisuje účinek léčby přípravkem Ecosin (registrovaná forma Pythium oligandrum pro zvířata) u kotěte britské kočky, u kterého byla klinicky a laboratorně diagnostikována dermatofytóza. Jako původce onemocnění bylo diagnostikováno Microsporum canis. Kotěti byla opakovaně aplikována koupel v přípravku Ecosin, který obsahuje živé kultury mykoparazitické houby Pythium oligandrum. Byly aplikovány tři celotělové koupele s týdenním odstupem a lokální aplikací přípravku, pokaždé druhý den po koupeli. Klinické vyšetření kotěte týden od ukončení aplikací již neprokázalo žádné příznaky dermatomykózy, negativní byly také kontrolní kultivace a trichoskopické vyšetření. Léčba přípravkem obsahujícím P. oligandrum byla bez jakýchkoli komplikací, bez nežádoucích i vedlejších účinků.
Presented case report describes therapeutical effect of biologic preparation Ecosin (registered form of mycoparasitic fungus Pythium oligandrum for animals) in a kitten of British shorthair cat, in which dermatophythosis has been diagnosed by means of clinical and laboratory examinations. Microsporum canis was identified as causal organism. The kitten was repeatedly treated by Ecosin in form of bath. Overall three baths were applied in a week interval and local administration of Ecosin was performed next day after each bath. No clinical symptoms were presented and both mycologic cultivation and trichoscopic examinations were negative after this medication. No adverse effect was recorded during and after treatment of the kitten by Ecosin.
- MeSH
- dermatitida * diagnóza terapie veterinární MeSH
- klinické laboratorní techniky MeSH
- kočky MeSH
- Microsporum * MeSH
- mykózy * terapie MeSH
- nemoci koček MeSH
- Pythium * MeSH
- zoonózy MeSH
- zvířata MeSH
- Check Tag
- kočky MeSH
- zvířata MeSH
- Publikační typ
- kazuistiky MeSH
Pythium oligandrum is a soil born free living oomycete able to parasitize fungi and oomycetes prey, including important plant and animals pathogens. Pythium oligandrum can colonize endophytically the root tissues of diverse plants where it induces plant defenses. Here we report the first long-read genome sequencing of a P. oligandrum strain sequenced by PacBio technology. Sequencing of genomic DNA loaded onto six SMRT cells permitted the acquisition of 913,728 total reads resulting in 112X genome coverage. The assembly and polishing of the genome sequence yielded180 contigs (N50 = 1.3 Mb; L50 = 12). The size of the genome assembly is 41.9 Mb with a longest contig of 2.7 Mb and 15,007 predicted protein-coding genes among which 95.25% were supported by RNAseq data, thus constituting a new Pythium genome reference. This data will facilitate genomic comparisons of Pythium species that are commensal, beneficial or pathogenic on plant, or parasitic on fungi and oomycete to identify key genetic determinants underpinning their diverse lifestyles. In addition comparison with plant pathogenic or zoopathogenic species will illuminate genomic adaptations for pathogenesis toward widely diverse hosts.
Objectives Dermatophytosis, commonly known as ringworm, is a superficial fungal skin disease and zoonosis. Pythium oligandrum is a micromycete with mycoparasitic properties that is used in agriculture to control fungal infections on plants. Formulations containing P oligandrum were also developed for the treatment of dermatophytoses, but only a small number of case studies have been published. In order to document the process in simplified conditions in vitro, we investigated the effectiveness of P oligandrum against three pathogenic dermatophytes common in domestic animals. Methods Cultures of the pathogens grown on nutrient media and experimentally infected cat hair were treated with P oligandrum preparations in therapeutic concentration and the changes were documented by microscopic videos and scanning electron microscopy. Results There was strong mycoparasitic activity of P oligandrum against Microsporum canis, Microsporum gypseum and Trichophyton mentagrophytes. Conclusions and relevance P oligandrum was demonstrated to be effective against three common causes of dermatophytosis in vitro.
- MeSH
- antifungální látky farmakologie MeSH
- kočky MeSH
- Microsporum účinky léků MeSH
- nemoci koček farmakoterapie mikrobiologie MeSH
- parazitické testy citlivosti veterinární MeSH
- Pythium * MeSH
- tinea farmakoterapie mikrobiologie veterinární MeSH
- Trichophyton účinky léků MeSH
- vlasy, chlupy mikrobiologie MeSH
- výsledek terapie MeSH
- zvířata MeSH
- Check Tag
- kočky MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Pythium oligandrum (Oomycota) is known for its strong mycoparasitism against more than 50 fungal and oomycete species. However, the ability of this oomycete to suppress and kill the causal agents of dermatophytoses is yet to be studied. We provide a complex study of the interactions between P. oligandrum and dermatophytes representing all species dominating in the developed countries. We assessed its biocidal potential by performing growth tests, on both solid and liquid cultivation media and by conducting a pilot clinical study. In addition, we studied the molecular background of mycoparasitism using expression profiles of genes responsible for the attack on the side of P. oligandrum and the stress response on the side of Microsporum canis. We showed that dermatophytes are efficiently suppressed or killed by P. oligandrum in the artificial conditions of cultivations media between 48 and 72 h after first contact. Significant intra- and interspecies variability was noted. Of the 69 patients included in the acute regimen study, symptoms were completely eliminated in 79% of the patients suffering from foot odour, hyperhidrosis disappeared in 67% of cases, clinical signs of dermatomycoses could no longer be observed in 83% of patients, and 15% of persons were relieved of symptoms of onychomycosis. Our investigations provide clear evidence that the oomycete is able to recognize and kill dermatophytes using recognition mechanisms that resemble those described in oomycetes attacking fungi infecting plants, albeit with some notable differences.
- MeSH
- antibióza * MeSH
- Arthrodermataceae růst a vývoj MeSH
- biologická terapie metody MeSH
- fyziologický stres MeSH
- lidé MeSH
- mikrobiální viabilita MeSH
- pilotní projekty MeSH
- Pythium růst a vývoj MeSH
- stanovení celkové genové exprese MeSH
- tinea terapie MeSH
- výsledek terapie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- bakteriální nálož MeSH
- biofilmy MeSH
- dolní končetina MeSH
- hojení ran * účinky léků MeSH
- lékové roztoky terapeutické užití MeSH
- lidé MeSH
- obvazy hydrokoloidní MeSH
- Pythium * MeSH
- rány a poranění mikrobiologie MeSH
- výsledek terapie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- abstrakt z konference MeSH
- MeSH
- antifungální látky terapeutické užití MeSH
- biologická terapie * metody využití MeSH
- biologické přípravky terapeutické užití MeSH
- hodnotící studie jako téma MeSH
- lidé MeSH
- mykózy farmakoterapie komplikace prevence a kontrola MeSH
- preventivní lékařství MeSH
- Pythium * chemie účinky léků MeSH
- statistika jako téma MeSH
- tinea pedis farmakoterapie prevence a kontrola terapie MeSH
- tinea * farmakoterapie prevence a kontrola MeSH
- výsledky a postupy - zhodnocení (zdravotní péče) MeSH
- Check Tag
- lidé MeSH
